Real-Time Label-Free Dynamic Cell Analysis

Summary

RTCA real-time detection of live cell activity

Principle

Through a special process, the microelectronic cell sensor chip is integrated into the bottom of the cell detection plate, which is used to construct a cell impedance detection sensing system for real-time, dynamic and quantitative tracking of changes in cell morphology and proliferation and differentiation.

When the cells growing on the surface of the microelectrode cause a change in the impedance of the interface of the walled electrode, this change is correlated with the real-time functional state of the cells, and the real-time dynamic impedance detection of the electrode can be used to obtain biological information related to the physiological function of the cells, including cell growth, extension, morphology, death and walling, and so on.

Appliance

Proliferation and Cytotoxicity
Migration & Infiltration
Tumor & Microenvironment - Co-Culture
Signaling Pathways
Tumor Immunity - Cellular Immunity

Operation method

Real-Time Label-Free Dynamic Cell Analysis

Materials and Instruments

【Instruments】xCELLigence or iCELLigence cell function analyzer and corresponding E-Plate test plate, ultra-clean bench, CO2 incubator, low-speed centrifuge, pipette, cell counting plate, optical microscope.

Move

1. Add culture medium to the E-Plate and measure the background impedance value.2、Collect and count the logarithmic cells, adjust the concentration of cell suspension, add a certain volume of cells to the E-Plate, and leave it in the ultra-clean bench at room temperature for 30min.3、Place the E-Plate plate with added cells on the test bench (the test bench is placed in the incubator beforehand), and carry out real-time dynamic cell proliferation detection.4. After the overnight assay, add the prepared gradient drug into each culture well and continue the real-time dynamic assay to obtain the compound-mediated cell effect curve and IC50 values at different time intervals.

Caveat

Real-time detection of cell proliferation and toxicity can be used to determine the critical time points of cell changes. The detected cell samples can be used for cell morphology observation, Western blot, Elisa, apoptosis and immunofluorescence, and other cell detection experiments.

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https://www.aladdinsci.com/