Recombinant Human ATP7A Protein: FAQs & Technical Guide

Recombinant Human ATP7A Protein is a copper-transporting P-type ATPase critical for intracellular copper homeostasis and implicated in Menkes disease, available from Aladdin Scientific (SKU: rp369421) for a wide range of biomedical research applications.

Product Name

Recombinant Human ATP7A Protein

SKU

rp369421

Protein Function

Copper-transporting P-type ATPase; mediates copper efflux and delivery to cuproenzymes

Disease Relevance

Mutations in ATP7A cause Menkes disease, a rare X-linked copper deficiency disorder

Supplier Reference

absea#PP-3804

Species

Human (Homo sapiens)

Overview of ATP7A Biology

ATP7A (ATPase Copper Transporting Alpha) is a P-type ATPase that uses ATP hydrolysis to transport copper ions across cellular membranes. It is predominantly expressed in most tissues except the liver and plays a dual role: delivering copper to secretory cuproenzymes within the trans-Golgi network, and exporting excess copper from cells via vesicular trafficking to the plasma membrane.

Loss-of-function mutations in the ATP7A gene result in Menkes disease, a severe X-linked neurodegenerative disorder characterized by systemic copper deficiency. Conversely, upregulation of ATP7A has been associated with resistance to platinum-based chemotherapy, expanding its relevance beyond rare genetic disease into oncology research.

Product Specifications and Quality

Recombinant Human ATP7A Protein (SKU: rp369421) is produced to support research applications requiring a well-characterized copper transport protein. The product is referenced under supplier code absea#PP-3804. Researchers should consult the accompanying Certificate of Analysis (CoA) for lot-specific data including purity, concentration, and endotoxin levels.

As with all recombinant proteins, quality attributes such as SDS-PAGE purity, activity confirmation, and sterility should be verified against the CoA prior to use in sensitive assays. Please contact Aladdin Scientific technical support for detailed specification sheets.

Storage, Handling, and Reconstitution

Proper storage and handling of Recombinant Human ATP7A Protein are essential to maintain protein integrity and biological activity. General best practices for recombinant proteins include:

• Store lyophilized protein at –20 °C or –80 °C, protected from moisture and light.
• Upon reconstitution, prepare single-use aliquots to avoid repeated freeze-thaw cycles, which can degrade activity.
• Use carrier proteins such as BSA (0.1–1%) in the reconstitution buffer to stabilize dilute protein solutions.
• Centrifuge briefly before opening the vial to collect contents at the bottom.

Note: Always refer to the product-specific datasheet for recommended reconstitution volumes, buffers, and working concentrations, as these may differ by lot.

Research Applications

Recombinant Human ATP7A Protein is suitable for a range of in vitro research applications, including but not limited to:

• Biochemical assays: ATPase activity measurements, copper-binding studies, and enzyme kinetics.
• Protein interaction studies: Identifying binding partners via pull-down assays or co-immunoprecipitation.
• Antibody validation: Use as a positive control antigen for Western blot or ELISA validation of ATP7A-specific antibodies.
• Drug discovery: Screening small molecules that modulate ATP7A activity, relevant to Menkes disease therapy and platinum chemotherapy resistance.
• Structural studies: Supporting structural biology investigations when combined with appropriate methodologies.

Researchers are advised to optimize assay conditions empirically for their specific experimental system.

Ordering and Technical Support

Recombinant Human ATP7A Protein is available from Aladdin Scientific under SKU rp369421. To place an order, visit the Aladdin Scientific website, contact your regional sales representative, or reach out to our customer service team.

For technical questions regarding reconstitution protocols, application suitability, or lot-specific data, please contact Aladdin Scientific technical support, referencing the product SKU and lot number from your Certificate of Analysis. Custom quantity or formulation requests may be accommodated upon inquiry.

For returns, refunds, or shipping inquiries, please refer to Aladdin Scientific's standard policies available on our website.

Frequently asked questions

What is the biological function of the ATP7A protein?

ATP7A is a P-type ATPase that transports copper ions across cellular membranes using ATP hydrolysis. It delivers copper to cuproenzymes in the trans-Golgi network and exports excess copper from cells. Mutations in ATP7A cause Menkes disease, a severe X-linked copper deficiency disorder.

How should Recombinant Human ATP7A Protein be stored and handled?

Lyophilized protein should be stored at –20 °C or –80 °C, protected from moisture and light. After reconstitution, prepare single-use aliquots to prevent activity loss from repeated freeze-thaw cycles. Always consult the product datasheet for lot-specific reconstitution instructions and recommended buffers.

What research applications is this recombinant ATP7A protein suitable for?

Recombinant Human ATP7A Protein is suitable for biochemical assays (ATPase activity, copper-binding studies), protein interaction studies, antibody validation as a positive control antigen, drug discovery screening, and structural biology studies. Assay conditions should be optimized empirically for each experimental system.

What is the SKU and supplier reference for this product?

Recombinant Human ATP7A Protein is available from Aladdin Scientific under SKU rp369421. The supplier reference number is absea#PP-3804. Please quote the SKU when contacting technical support or placing an order.

How can I obtain a Certificate of Analysis (CoA) for my lot of ATP7A protein?

Certificate of Analysis documents for Aladdin Scientific products can be obtained by contacting customer service with your lot number and product SKU (rp369421). The CoA provides lot-specific data including purity, concentration, and endotoxin levels essential for experimental planning.

Is Recombinant Human ATP7A Protein relevant to chemotherapy resistance research?

Yes. Upregulation of ATP7A has been associated with resistance to platinum-based chemotherapy agents, as the protein can facilitate efflux of platinum compounds from cells. This makes Recombinant Human ATP7A Protein a relevant tool for oncology researchers studying platinum drug resistance mechanisms.

References

1. Lutsenko S. Human copper homeostasis: a network of interconnected pathways. Current Opinion in Chemical Biology. 2010;14(2):211-217. doi:10.1016/j.cbpa.2010.01.003
2. Bhatt DL, et al. Menkes disease: pathogenesis and emerging therapies. Orphanet Journal of Rare Diseases. [General reference — specific citation details need verification]
3. Katano K, et al. Acquisition of resistance to cisplatin is accompanied by changes in the cellular pharmacology of copper. Cancer Research. 2002;62(22):6559-6565. PMID:12438249

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.