Recombinant Human ATXN2L Protein: Key FAQs & specs

Recombinant Human ATXN2L Protein is a research-grade recombinant protein representing the human ataxin-2-like protein, a conserved RNA-binding factor involved in mRNA regulation, stress granule assembly, and signalling pathways relevant to neurological and cellular stress research.

Product Name

Recombinant Human ATXN2L Protein

SKU

rp374250

Protein Family

Ataxin-2 family; contains LSm and PAM2 domains

Biological Function

RNA-binding protein involved in mRNA stability, translational regulation, and stress granule formation

Research Applications

Functional assays, protein interaction studies, antibody validation, structural studies

Storage Recommendation

Avoid repeated freeze-thaw cycles; store at -20°C or -80°C as recommended in product datasheet

Supplier

Aladdin Scientific

What Is Recombinant Human ATXN2L Protein?

ATXN2L (Ataxin-2-Like protein) is a paralog of Ataxin-2 (ATXN2) and belongs to the conserved ataxin-2 protein family. It contains an LSm (Like-Sm) domain and a PAM2 (PABP-interacting motif 2) domain, which mediate interactions with RNA and poly(A)-binding proteins, respectively. These structural features position ATXN2L as a key regulator of mRNA metabolism, including mRNA stability and translational control.

Recombinant Human ATXN2L Protein (SKU: rp374250) is produced using recombinant expression technology to deliver a research-grade protein suitable for in vitro biochemical and cell biology experiments. It enables researchers to study ATXN2L function without relying solely on endogenous expression levels.

Biological Role and Research Significance of ATXN2L

ATXN2L plays multifaceted roles in cellular physiology. It is a component of stress granules — cytoplasmic RNA-protein condensates that form transiently during cellular stress conditions such as oxidative stress, heat shock, or viral infection. By participating in stress granule assembly and disassembly, ATXN2L contributes to the cell's adaptive response to stress.

Beyond stress biology, ATXN2L interacts with mTORC1 signalling components and has been implicated in the regulation of cell growth and metabolic pathways. Its paralogue ATXN2 is associated with spinocerebellar ataxia type 2 (SCA2) and amyotrophic lateral sclerosis (ALS); ATXN2L shares structural homology and may have overlapping functional roles relevant to neurodegeneration research.

Recombinant ATXN2L protein facilitates biochemical dissection of these pathways, supporting protein–protein interaction assays, pull-down experiments, and in vitro reconstitution of stress granule components.

Recommended Applications and Handling Guidelines

Recombinant Human ATXN2L Protein (rp374250) is suitable for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: Use in co-immunoprecipitation (Co-IP) or pull-down assays to map ATXN2L interactome.
• Antibody validation: Serve as a positive control antigen for validating anti-ATXN2L antibodies in Western blotting or ELISA.
• Functional biochemical assays: Assess RNA-binding activity or domain-specific interactions using purified recombinant protein.
• Structural studies: Use in biophysical characterisation experiments including size-exclusion chromatography or dynamic light scattering.

For handling, minimise freeze-thaw cycles by aliquoting the protein upon first use. Refer to the product datasheet for specific buffer composition, recommended working concentrations, and storage conditions. Contact Aladdin Scientific technical support if additional guidance is required.

Quality, Purity and Regulatory Considerations

Recombinant proteins from Aladdin Scientific are manufactured under quality-controlled processes to ensure batch-to-batch consistency. Typical quality attributes assessed for recombinant proteins include SDS-PAGE purity, endotoxin level, and biological activity (where applicable). Users are advised to consult the certificate of analysis (CoA) supplied with each lot for lot-specific data.

ATXN2L recombinant protein (rp374250) is intended for research use only and is not approved for diagnostic, therapeutic, or clinical applications. Researchers should follow institutional biosafety guidelines when handling recombinant proteins.

For detailed purity specifications, expression host information, and tag details, refer to the product datasheet available on the Aladdin Scientific website or contact the technical support team directly.

Ordering and Support Information

Recombinant Human ATXN2L Protein is available from Aladdin Scientific under SKU rp374250. To place an order, visit the Aladdin Scientific website and search by product name or SKU, or contact your regional sales representative.

For technical enquiries — including advice on experimental design, appropriate positive controls, or compatibility with specific assay kits — please reach out to the Aladdin Scientific technical support team. Custom quantities, bulk pricing, or special formulation requests may be accommodated; contact the sales team for details.

Returns, refunds, and shipping policies are governed by Aladdin Scientific's standard terms and conditions, available on the company website. Ensure that cold-chain packaging integrity is verified upon receipt of temperature-sensitive products.

Frequently asked questions

What is the biological function of ATXN2L?

ATXN2L is an RNA-binding protein that regulates mRNA stability and translation. It is a key component of cytoplasmic stress granules, which form in response to cellular stressors such as oxidative stress or heat shock, and it interacts with components of the mTORC1 signalling pathway.

What research applications is Recombinant Human ATXN2L Protein suitable for?

This protein is suitable for protein–protein interaction studies (e.g., Co-IP, pull-down assays), antibody validation as a positive control antigen, in vitro RNA-binding assays, and biophysical characterisation experiments. It is intended for research use only and is not for diagnostic or therapeutic use.

How should Recombinant Human ATXN2L Protein be stored to maintain stability?

To maintain protein stability, avoid repeated freeze-thaw cycles by aliquoting the protein into single-use volumes upon first thaw. Store at -20°C or -80°C as specified in the product datasheet. Always refer to the certificate of analysis and datasheet supplied with your specific lot for authoritative storage instructions.

Is ATXN2L related to any neurological diseases?

ATXN2L is a structural and functional paralog of ATXN2, which is directly linked to spinocerebellar ataxia type 2 (SCA2) and has been implicated as a modifier of TDP-43 toxicity in ALS research. While ATXN2L itself has not been as directly linked to these diseases as ATXN2, its structural homology makes it a relevant subject of study in neurodegeneration and RNA biology research.

Where can I find purity and lot-specific data for SKU rp374250?

Lot-specific purity and quality data are provided in the certificate of analysis (CoA) included with each shipment. The product datasheet, which contains expression host, tag information, formulation, and recommended storage conditions, is available on the Aladdin Scientific website. Contact Aladdin Scientific technical support for additional information.

References

1. Nonhoff U, et al. Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules. Molecular Biology of the Cell, 2007.
2. Bakthavachalu B, et al. RNP-Granule Assembly via Ataxin-2 Disordered Domains Is Required for Long-Term Memory and Neurodegeneration. Neuron, 2018.
3. Elden AC, et al. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Nature, 2010.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.