Recombinant Human CHMP4B Protein: FAQs & Key Facts

Recombinant Human CHMP4B Protein is a key ESCRT-III pathway component produced recombinantly for use in biochemical, cell biology, and protein interaction research applications.

Product Name

Recombinant Human CHMP4B Protein

SKU

rp371258

Protein Family

ESCRT-III / Charged Multivesicular Body Protein (CHMP) family

Species Origin

Human (Homo sapiens)

Primary Function

Involved in multivesicular body (MVB) biogenesis, membrane abscission, and cytokinesis via the ESCRT-III complex

Supplier Reference

absea#PP-5431

What Is CHMP4B and Why Is It Important?

CHMP4B (Charged Multivesicular Body Protein 4B), also known as SNF7-2, is a core subunit of the ESCRT-III (Endosomal Sorting Complexes Required for Transport III) machinery. It plays a critical role in membrane remodeling processes including multivesicular body (MVB) biogenesis, viral budding, nuclear envelope reformation, and the final abscission step of cytokinesis.

CHMP4B polymerizes into helical filaments that constrict membrane necks from the cytoplasmic face, making it a central effector of ESCRT-III-driven membrane fission. Dysregulation of CHMP4B has been implicated in neurodegenerative diseases, cancer biology, and inherited cataracts, underscoring its broad biomedical relevance.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human CHMP4B Protein (SKU: rp371258) is produced using recombinant expression systems to ensure consistent batch-to-batch quality for demanding research applications. The product is referenced under supplier code absea#PP-5431.

Researchers are advised to consult the official product datasheet available on the Aladdin Scientific website or by contacting technical support for the most current information on purity grade, expression host, tag configuration, molecular weight, formulation buffer, and concentration. These parameters are critical for experimental design and should be verified prior to use.

Recommended Applications

Recombinant Human CHMP4B Protein is suitable for a range of in vitro and biochemical research applications. Common uses include:

• Protein–protein interaction studies: Investigating CHMP4B binding partners within the ESCRT-III complex or with upstream ESCRT-II components and downstream VPS4.
• Biochemical assays: Enzyme-linked immunosorbent assays (ELISA) for antibody validation, Western blot standards, and dot blot applications.
• Structural and biophysical studies: In vitro reconstitution of ESCRT-III filaments for electron microscopy or light scattering experiments.
• Cell-free functional assays: Reconstituting membrane remodeling or MVB sorting activities in defined systems.

Specific validated applications are listed on the product datasheet. Users should perform application-specific optimization and titration experiments.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are essential to maintain activity and prevent degradation. General best practices for recombinant proteins of this class include:

• Store at –80 °C for long-term preservation; –20 °C may be acceptable for short-term storage depending on formulation.
• Avoid repeated freeze–thaw cycles. Aliquot the protein upon receipt to minimize freeze–thaw events.
• Keep the protein on ice during experimental use.
• Centrifuge briefly before opening the vial to recover any material from the lid or walls.

Note: Exact recommended storage conditions, shelf life, and stability data for SKU rp371258 should be confirmed from the official product datasheet, as formulation specifics govern optimal storage temperature and acceptable freeze–thaw cycles.

Ordering, Pricing, and Technical Support

Recombinant Human CHMP4B Protein (SKU: rp371258) can be ordered directly through the Aladdin Scientific website or by contacting your regional Aladdin Scientific sales representative. For bulk quantities, custom formulations, or institutional pricing, please reach out to the sales team.

Technical questions regarding experimental design, compatibility, or troubleshooting can be directed to Aladdin Scientific's technical support team. When contacting support, please provide your SKU (rp371258), lot number, and a description of your experimental setup for the most efficient assistance.

Returns and refunds are handled in accordance with Aladdin Scientific's standard return policy. Please consult the Help and Customer Service section of the Aladdin Scientific website for details on eligibility, timelines, and procedures.

Frequently asked questions

What is the biological function of CHMP4B?

CHMP4B is a core subunit of the ESCRT-III complex that mediates membrane remodeling events including multivesicular body (MVB) formation, cytokinetic abscission, nuclear envelope sealing, and viral budding. It polymerizes into helical filaments that constrict and sever membrane necks from the cytoplasmic side.

What applications is Recombinant Human CHMP4B Protein validated for?

Recombinant Human CHMP4B Protein is commonly used in protein–protein interaction studies, ELISA-based antibody validation, Western blot standards, and in vitro reconstitution of ESCRT-III assemblies. For the full list of validated applications specific to SKU rp371258, please refer to the official product datasheet or contact Aladdin Scientific technical support.

How should Recombinant Human CHMP4B Protein be stored to maintain activity?

As a general best practice for recombinant proteins, store at –80 °C for long-term use, aliquot upon receipt to avoid repeated freeze–thaw cycles, and keep on ice during experiments. Always confirm the exact storage conditions from the product datasheet for SKU rp371258, as formulation-specific requirements may apply.

Can Recombinant Human CHMP4B Protein be used for structural studies?

CHMP4B has been extensively studied structurally due to its role in ESCRT-III filament assembly. Recombinant CHMP4B protein is suitable for in vitro reconstitution experiments including electron microscopy and biophysical characterization of filament assembly. Please verify suitability for your specific structural application with the product datasheet or technical support.

How do I place an order for SKU rp371258, and what are the available sizes?

You can order Recombinant Human CHMP4B Protein (rp371258) directly through the Aladdin Scientific website or by contacting your regional sales representative. For information on available sizes, pricing, and bulk order discounts, please visit the product page or contact Aladdin Scientific sales and customer service.

Is Recombinant Human CHMP4B Protein suitable for use as a Western blot standard?

Recombinant proteins are commonly employed as positive controls or molecular weight standards in Western blot experiments. Recombinant Human CHMP4B Protein can serve this role for researchers studying CHMP4B expression levels. Confirm the specific validated use from the product datasheet and ensure the tag or formulation is compatible with your detection system.

References

1. Henne WM, Buchkovich NJ, Emr SD. The ESCRT pathway. Dev Cell. 2011;21(1):77–91. doi:10.1016/j.devcel.2011.05.015
2. Hanson PI, Cashikar A. Multivesicular body morphogenesis. Annu Rev Cell Dev Biol. 2012;28:337–362. doi:10.1146/annurev-cellbio-092910-154152
3. Bhaskara RM, et al. Curvature induction and membrane remodeling by FAM134B reticulon homology domain assist selective ER-phagy. Nat Commun. 2019. [General ESCRT-III structural reference — verify relevance to CHMP4B specifically]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.