Recombinant Human GALNT7 Protein: FAQs & Technical Guide

Recombinant Human GALNT7 Protein is a research-grade recombinant enzyme representing the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7, a key initiating enzyme in mucin-type O-linked glycosylation of proteins.

Product Name

Recombinant Human GALNT7 Protein

SKU

rp365110

Gene Name

GALNT7 (also known as GalNAc-T7)

Protein Family

Polypeptide GalNAc-transferase family (GALNT family)

Biological Function

Initiates mucin-type O-linked glycosylation by transferring GalNAc from UDP-GalNAc to serine and threonine residues on target proteins

Species

Human (Homo sapiens)

Application Areas

Enzyme activity assays, substrate specificity studies, glycoprotein biosynthesis research, drug target validation

What Is GALNT7 and Why Is It Important in Research?

GALNT7 (UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7) is a member of the large GALNT enzyme family responsible for initiating the first step of mucin-type O-linked glycosylation. This post-translational modification involves the transfer of N-acetylgalactosamine (GalNAc) from the sugar donor UDP-GalNAc onto serine or threonine residues of acceptor proteins, catalyzed in the Golgi apparatus.

O-glycosylation plays critical roles in cell adhesion, immune recognition, receptor signaling, and protein stability. GALNT7 has been implicated in cancer biology, with studies suggesting altered expression in various tumor types. As a research tool, Recombinant Human GALNT7 Protein (rp365110) enables scientists to study substrate specificity, glycosylation site mapping, and enzyme kinetics in a controlled in vitro setting.

Protein Specifications and Quality Assurance

Recombinant Human GALNT7 Protein (rp365110) is produced to research-grade standards. The following specifications are generally applicable to recombinant GALNT family proteins of this type; buyers should confirm exact lot-specific data on the product datasheet or certificate of analysis (CoA):

• Expression System: Typically expressed in a mammalian or baculovirus/insect cell system to support proper folding and post-translational modifications required for enzyme activity. Please confirm the specific host system on the product CoA.
• Purity: Research-grade recombinant proteins in this class are generally >85% pure as assessed by SDS-PAGE. Consult the CoA for lot-specific purity data.
• Formulation: Supplied in a buffered solution or as a lyophilized powder; exact formulation is provided on the product datasheet.
• Tag Information: May include an affinity purification tag (e.g., His-tag) to facilitate purification; confirm tag position and sequence on the datasheet.

All lots are subject to quality control testing prior to release. Contact Aladdin Scientific technical support for lot-specific CoA documentation.

Storage, Handling, and Reconstitution

Proper storage and handling are essential to maintain the activity and integrity of Recombinant Human GALNT7 Protein. General best-practice guidelines for recombinant enzyme proteins of this class are as follows:

• Storage Temperature: Store at –20 °C or –80 °C for long-term storage. Avoid repeated freeze-thaw cycles, which can denature the enzyme and reduce activity.
• Aliquoting: Upon first thaw, aliquot the protein into single-use volumes to minimize freeze-thaw damage. Store working aliquots at 4 °C for short-term use (typically up to 1 week).
• Reconstitution (lyophilized form): If supplied as a lyophilized powder, briefly centrifuge the vial before opening. Reconstitute in sterile, deionized water or the buffer recommended on the datasheet to the desired concentration. Allow the solution to equilibrate for 10–15 minutes at room temperature with gentle mixing; avoid vigorous vortexing.
• Carrier Protein: Addition of 0.1% BSA (bovine serum albumin) to the reconstitution or storage buffer may help stabilize dilute protein solutions.

Note: Always refer to the lot-specific datasheet supplied with the product for definitive storage and reconstitution instructions, as formulations may vary between lots.

Recommended Applications and Experimental Use

Recombinant Human GALNT7 Protein is suitable for a range of in vitro biochemical and cell-free research applications, including but not limited to:

• In Vitro Glycosylation Assays: Use GALNT7 to transfer GalNAc onto peptide or protein substrates in the presence of UDP-GalNAc. Reaction products can be analyzed by mass spectrometry, lectin blotting, or radiolabeled sugar incorporation assays.
• Substrate Specificity Studies: Determine which peptide sequences or proteins serve as preferred substrates for GALNT7, contributing to glycosylation site prediction and mapping.
• Enzyme Kinetics: Measure Km and Vmax values for GALNT7 with defined peptide substrates to characterize catalytic efficiency.
• Drug Discovery and Inhibitor Screening: Use as a biochemical target to identify small-molecule inhibitors of GALNT7, relevant to cancer and other disease areas where O-glycosylation is dysregulated.
• Structural and Functional Studies: Suitable as a reference protein in structural biology experiments or interaction studies with known binding partners.

This product is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or clinical use.

Ordering, Shipping, and Support

Recombinant Human GALNT7 Protein is available from Aladdin Scientific under SKU rp365110. The following information applies to ordering and logistics:

• How to Order: Place orders directly through the Aladdin Scientific website, by contacting your regional sales representative, or via your institution's approved procurement channels.
• Shipping Conditions: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Upon receipt, inspect the package immediately and store the product according to the datasheet instructions.
• Lead Time: Standard lead times apply; contact Aladdin Scientific customer service for current stock availability and estimated delivery timelines.
• Technical Support: For application-specific questions, reconstitution guidance, or CoA requests, contact Aladdin Scientific technical support. Reference SKU rp365110 in all correspondence.
• Returns and Replacements: If a product arrives damaged or does not meet specifications stated on the CoA, contact Aladdin Scientific customer service within the applicable returns window. Please retain all original packaging and documentation.

Frequently asked questions

What is the biological function of GALNT7?

GALNT7 is a Golgi-localized glycosyltransferase that catalyzes the initial step of mucin-type O-linked glycosylation by transferring GalNAc from UDP-GalNAc to serine and threonine residues on target proteins. This modification influences protein folding, stability, and cell-surface interactions. Dysregulation of GALNT7 expression has been associated with cancer progression in several tumor types.

What applications is Recombinant Human GALNT7 Protein (rp365110) validated for?

This protein is suitable for in vitro glycosylation assays, substrate specificity studies, enzyme kinetics measurements, and small-molecule inhibitor screening. It may also be used as a reference protein in structural biology and protein interaction studies. Please consult the product datasheet for the confirmed list of validated applications for the specific lot.

How should I store and reconstitute Recombinant Human GALNT7 Protein?

For long-term storage, keep the protein at –20 °C or –80 °C and avoid repeated freeze-thaw cycles by aliquoting into single-use volumes upon first thaw. If the product is lyophilized, reconstitute with sterile deionized water or the recommended buffer as indicated on the datasheet, and mix gently. Adding 0.1% BSA to the buffer can help stabilize dilute solutions. Always refer to the lot-specific datasheet for definitive instructions.

Is Recombinant Human GALNT7 Protein suitable for clinical or diagnostic use?

No. Recombinant Human GALNT7 Protein (rp365110) is intended for research use only (RUO) and has not been evaluated or approved for use in diagnostic procedures, therapeutic applications, or clinical settings. It should only be used by qualified research personnel in a laboratory environment.

How do I request a Certificate of Analysis (CoA) for rp365110?

You can request a lot-specific Certificate of Analysis for rp365110 by contacting Aladdin Scientific customer service or technical support, providing your lot number and order information. CoAs may also be available for download on the product page of the Aladdin Scientific website.

What expression system is used to produce Recombinant Human GALNT7 Protein?

Recombinant GALNT family proteins typically require eukaryotic expression systems—such as mammalian cells or baculovirus-infected insect cells—to achieve proper folding and glycosylation-dependent activity. Please confirm the exact expression system used for rp365110 by reviewing the product datasheet or contacting Aladdin Scientific technical support, as this information is lot-specific.

References

1. Bennett EP, Mandel U, Clausen H, Gerken TA, Fritz TA, Tabak LA. Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family. Glycobiology. 2012;22(6):736-756. doi:10.1093/glycob/cwr182
2. Halim A, Larsen ISB, Neubert P, Joshi HJ, Petersen BL, Vakhrushev SY, Strahl S, Clausen H. Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast. Proc Natl Acad Sci USA. 2015;112(51):15648-15653.
3. Munkley J, Elliott DJ. Hallmarks of glycosylation in cancer. Oncotarget. 2016;7(23):35478-35489. doi:10.18632/oncotarget.8155

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.