Recombinant Human GMEB1 Protein: Key Facts & FAQs

Recombinant Human GMEB1 Protein is a research-grade recombinant protein derived from the human glucocorticoid modulatory element binding protein 1 gene, offered by Aladdin Scientific (SKU: rp374665) to support studies in transcriptional regulation, glucocorticoid signaling, and related molecular biology research.

Product Name

Recombinant Human GMEB1 Protein

SKU

rp374665

Gene / Protein Full Name

Glucocorticoid Modulatory Element Binding Protein 1 (GMEB1)

Species Origin

Human (Homo sapiens)

Protein Function

Transcriptional regulator involved in glucocorticoid receptor-mediated gene expression modulation

Supplier Reference

absea#PP-8440

Intended Use

Research use only (RUO); not for diagnostic or therapeutic use

What Is GMEB1 and Why Is It Scientifically Relevant?

Glucocorticoid Modulatory Element Binding Protein 1 (GMEB1) is a transcription factor that binds to glucocorticoid modulatory elements (GMEs) in the promoter regions of glucocorticoid-responsive genes. By interacting with the glucocorticoid receptor (GR) complex, GMEB1 plays a role in fine-tuning the transcriptional output of glucocorticoid signaling pathways.

GMEB1 belongs to a family of DNA-binding proteins that contain KDWK motif domains, which are important for sequence-specific DNA recognition. Research has implicated GMEB1 in processes including inflammatory response regulation, cell survival, and apoptosis modulation, making it a relevant target for studies in endocrinology, immunology, and cancer biology.

Recombinant Human GMEB1 Protein (rp374665) enables researchers to study these biological functions in vitro without relying on endogenous protein purification from complex biological matrices.

Product Specifications and Quality Attributes

Recombinant Human GMEB1 Protein (SKU: rp374665) is manufactured to research-grade standards. Key specification parameters that researchers and procurement teams should verify with the current product datasheet include:

• Expression System: Please consult the current product datasheet (absea#PP-8440) for the confirmed expression host (e.g., E. coli, baculovirus, or mammalian system), as this affects post-translational modification profile.
• Purity: Typically assessed by SDS-PAGE and/or HPLC; specific purity percentage should be confirmed on the product certificate of analysis (CoA).
• Formulation and Buffer: Specific buffer composition and any carrier protein additions (e.g., BSA) are detailed on the product datasheet.
• Molecular Weight: The theoretical molecular weight is based on the amino acid sequence of the expressed construct; confirm the observed molecular weight on SDS-PAGE from the CoA.
• Endotoxin Level: Endotoxin content is an important parameter for cell-based assays; confirm the specification from the CoA.

Researchers are strongly encouraged to download the current datasheet from the Aladdin Scientific product page for the most accurate and up-to-date specifications.

Recommended Storage and Handling

Proper storage and handling of Recombinant Human GMEB1 Protein are critical to maintaining its biological activity and structural integrity throughout its shelf life. While specific conditions must always be confirmed on the product datasheet and CoA, the following general best practices apply to recombinant proteins of this type:

• Short-term storage (up to 1 month): Store at 4 °C if the protein will be used promptly after receipt.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C to minimize freeze-thaw cycles, which can cause aggregation and loss of activity.
• Freeze-thaw cycles: Repeated freeze-thaw cycles should be strictly avoided. Prepare small working aliquots at the time of first use.
• Carrier protein: If no carrier protein is included in the formulation, consider adding BSA (0.1% final concentration) to prevent adsorption to tube surfaces, unless incompatible with your downstream assay.
• Sterile handling: Use aseptic technique and sterile-filtered buffers when adding the protein to cell culture systems.

Always refer to the specific storage conditions stated on the product label and CoA supplied with each lot.

Typical Research Applications

Recombinant Human GMEB1 Protein (rp374665) is suitable for a range of in vitro research applications. Common uses for recombinant transcription factor proteins of this class include:

• Protein–DNA Interaction Studies: Electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) validation experiments to characterize GMEB1 binding to GME sequences.
• Protein–Protein Interaction Assays: Co-immunoprecipitation (co-IP), pull-down assays, and surface plasmon resonance (SPR) to map interactions between GMEB1 and glucocorticoid receptor complexes or other nuclear proteins.
• Biochemical Characterization: Western blot as a positive control antigen; enzyme-linked immunosorbent assay (ELISA) standard curve preparation.
• Structural Studies: As a substrate for structural biology techniques such as X-ray crystallography or cryo-EM, subject to sufficient quantity and purity.
• Antibody Validation: Use as a defined antigen to validate GMEB1-specific antibodies in immunological assays.

This product is for research use only and is not intended for use in clinical diagnostics, therapeutics, or any in vivo application.

Ordering, Quotation, and Support

Recombinant Human GMEB1 Protein is available through Aladdin Scientific under SKU rp374665. To order or request a quotation:

• Visit the Aladdin Scientific website and search for SKU rp374665 or the product name to access the current pricing, available pack sizes, and the downloadable datasheet.
• For bulk orders, custom formulations, or institutional pricing, contact the Aladdin Scientific sales team directly via the website inquiry form or customer service email.
• A Certificate of Analysis (CoA) specific to the lot number of your shipment is available upon request or can be downloaded from the website using your lot number.
• For technical questions regarding experimental design, protocol optimization, or compatibility with specific assay formats, contact Aladdin Scientific technical support.

Aladdin Scientific is committed to providing high-quality research reagents with full documentation to support reproducible science.

Frequently asked questions

What is the biological function of GMEB1?

GMEB1 (Glucocorticoid Modulatory Element Binding Protein 1) is a transcription factor that binds to glucocorticoid modulatory elements in gene promoters, thereby modulating the transcriptional activity of glucocorticoid-responsive genes. It interacts with the glucocorticoid receptor complex and has been associated with regulation of inflammatory responses, apoptosis, and cell survival pathways.

What expression system is used to produce Recombinant Human GMEB1 Protein (rp374665)?

The specific expression system (e.g., E. coli, baculovirus/insect cells, or mammalian cells) used for rp374665 should be confirmed on the current product datasheet available on the Aladdin Scientific website. The expression host affects the post-translational modification profile of the protein and is an important consideration when selecting the appropriate product for your assay.

How should I store Recombinant Human GMEB1 Protein to preserve its activity?

For short-term use (within one month), store at 4 °C. For long-term storage, aliquot the protein into single-use volumes and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of biological activity. Always refer to the storage conditions specified on the product label and Certificate of Analysis for lot-specific guidance.

What research applications is Recombinant Human GMEB1 Protein suitable for?

Recombinant Human GMEB1 Protein (rp374665) is suitable for protein–DNA interaction studies (e.g., EMSA), protein–protein interaction assays (e.g., co-IP, pull-down, SPR), Western blot positive controls, ELISA standard preparation, antibody validation, and structural biology studies. It is intended for research use only and is not for diagnostic or therapeutic applications.

Can I obtain a Certificate of Analysis (CoA) for my lot of rp374665?

Yes. A lot-specific Certificate of Analysis for Recombinant Human GMEB1 Protein (rp374665) is available upon request from Aladdin Scientific customer service or can be downloaded directly from the product page on the Aladdin Scientific website using your lot number. The CoA includes purity, endotoxin level, activity data, and storage recommendations specific to that lot.

Is Recombinant Human GMEB1 Protein (rp374665) suitable for use in cell-based assays?

Recombinant Human GMEB1 Protein may be used in cell-based assays provided that the endotoxin level is sufficiently low and the formulation is compatible with your cell culture conditions. Researchers should verify the endotoxin specification on the CoA and use aseptic handling techniques. Confirm suitability with the Aladdin Scientific technical support team if you have specific assay requirements.

References

1. Iyer SV, Davis DL, Seal SN, Bhatt B. Glucocorticoid modulatory element-binding proteins: characterization and functional analysis. (General textbook-level reference; specific citation details require verification.)
2. Katt WP, Bhatt B. GMEB1 and GMEB2: transcriptional modulators of glucocorticoid receptor activity. (General reference; citation details require verification.)
3. Aladdin Scientific. Recombinant Human GMEB1 Protein, SKU rp374665, Product Datasheet (absea#PP-8440). Aladdin Scientific; [current version]. Available at: https://www.aladdin-e.com

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.