Recombinant Human GPR158 Protein: FAQs & Technical Guide

Recombinant Human GPR158 Protein is a research-grade recombinant form of the human orphan G protein-coupled receptor 158, produced to support studies in signal transduction, neuroscience, and drug discovery.

Product Name

Recombinant Human GPR158 Protein

SKU

rp368046

Protein Family

Class C G protein-coupled receptor (GPCR), orphan receptor

Species Origin

Human (Homo sapiens)

Application

Signal transduction research, neuroscience studies, drug discovery, biochemical assays

Format

Recombinant protein (specific tag, purity, and formulation details subject to datasheet confirmation)

What Is GPR158 and Why Is It Scientifically Relevant?

GPR158 (G Protein-Coupled Receptor 158) is an orphan member of the Class C GPCR superfamily, meaning its endogenous ligand(s) have not been fully characterized. It shares structural homology with metabotropic glutamate receptors (mGluRs) and GABA_B receptors, featuring a large extracellular domain and a characteristic seven-transmembrane topology.

GPR158 is highly expressed in the brain, particularly in regions associated with mood regulation, stress response, and cognitive function such as the prefrontal cortex and cerebellum. Emerging research has implicated GPR158 in major depressive disorder, schizophrenia, and other neuropsychiatric conditions, making it a target of significant interest in both basic neuroscience and translational drug discovery programs.

Recombinant Human GPR158 Protein provides researchers with a well-defined, reproducible reagent to investigate receptor structure, potential ligand interactions, and downstream signaling mechanisms without the variability inherent in cell-based or tissue-derived preparations.

Expression System, Purity, and Formulation

Recombinant proteins can be produced in a variety of expression systems, each with distinct advantages. For complex membrane-associated proteins such as GPCRs, mammalian or insect cell (baculovirus) systems are generally preferred because they support the post-translational modifications, proper folding, and membrane topology critical for functional integrity.

Specific details regarding the expression host, affinity tag, purity grade, buffer composition, and concentration for SKU rp368046 are provided on the official product datasheet. Researchers are strongly encouraged to consult the current datasheet prior to experimental use, as formulation details are subject to lot-to-lot optimization.

If you require a custom formulation, endotoxin testing data, or a certificate of analysis (CoA) for a specific lot, please contact Aladdin Scientific's technical support team.

Storage, Handling, and Stability Recommendations

Proper storage and handling of recombinant proteins are essential to maintain activity and avoid degradation. General best practices for recombinant proteins of this class include:

• Short-term storage (≤1 week): Store at 4°C in the supplied buffer.
• Long-term storage: Aliquot into single-use volumes and store at −20°C or −80°C to minimize freeze-thaw cycles.
• Avoid repeated freeze-thaw: Each cycle can reduce protein activity and promote aggregation.
• Carrier protein: If the protein is supplied at low concentration, addition of a stabilizing carrier such as BSA (0.1%) may be recommended — consult the datasheet.

Always refer to the product-specific datasheet for authoritative storage conditions, as recommendations may differ by formulation and lot. Specific stability data for rp368046 should be confirmed via the CoA or by contacting technical support.

Research Applications and Experimental Considerations

Recombinant Human GPR158 Protein is suitable for a range of biochemical and cell-free research applications, including but not limited to:

• Ligand binding assays: Screening small molecules, peptides, or lipid mediators for binding to GPR158 extracellular or intracellular domains.
• Protein–protein interaction studies: Investigating interactions with G proteins, scaffolding proteins (e.g., RGS proteins), or other signaling partners.
• Structural studies: Supporting crystallography, cryo-EM, or biophysical characterization efforts.
• Antibody validation: Serving as a positive control antigen for antibodies targeting GPR158.
• Enzyme-linked immunosorbent assays (ELISA): As a standard or coating antigen.

Researchers should consider that full-length transmembrane GPCRs present unique challenges in recombinant production and functional assays. Confirm the domain coverage (e.g., extracellular domain only vs. full-length) from the datasheet to ensure suitability for your specific application.

Ordering, Quality, and Technical Support

Recombinant Human GPR158 Protein is available from Aladdin Scientific under SKU rp368046. To place an order or request a quote, visit the product page on the Aladdin Scientific website or contact your regional sales representative.

Quality documentation available upon request:

• Certificate of Analysis (CoA) specific to your lot number
• Safety Data Sheet (SDS)
• Endotoxin test results
• Purity data (e.g., SDS-PAGE, SEC-HPLC)

For technical inquiries regarding experimental design, compatibility, or troubleshooting, Aladdin Scientific's scientific support team is available via the website contact form or by email. Bulk quantities and custom formulations may be available upon request.

Frequently asked questions

What is GPR158 and what makes it an orphan receptor?

GPR158 is a Class C G protein-coupled receptor that is structurally related to metabotropic glutamate receptors. It is classified as an orphan receptor because its endogenous ligand(s) have not yet been conclusively identified and validated, making it an active area of investigation in pharmacology and neuroscience.

What expression system is used to produce Recombinant Human GPR158 Protein (rp368046)?

The specific expression system for rp368046 is detailed in the product datasheet. For complex receptors like GPCRs, mammalian or insect (baculovirus) cell systems are commonly employed to ensure proper folding and post-translational modifications. Please consult the current datasheet or contact technical support for lot-specific information.

How should I store Recombinant Human GPR158 Protein to maintain its activity?

As a general guideline, recombinant proteins should be aliquoted into single-use volumes upon receipt and stored at −20°C or −80°C for long-term stability, avoiding repeated freeze-thaw cycles. Short-term storage at 4°C is acceptable for up to one week. Always defer to the specific storage instructions on the product datasheet for rp368046.

What research applications is Recombinant Human GPR158 Protein validated for?

Recombinant Human GPR158 Protein is suitable for applications including ligand binding assays, protein-protein interaction studies, structural biology experiments, antibody validation, and use as an ELISA antigen. Validated applications for the specific lot of rp368046 are listed on the product datasheet — confirm domain coverage and activity data before use in functional assays.

Can I request a Certificate of Analysis or custom formulation for rp368046?

Yes. A Certificate of Analysis (CoA) specific to your lot number, along with Safety Data Sheets and purity data, can be requested from Aladdin Scientific's technical support team. Bulk quantities and custom formulations (e.g., alternative buffers, endotoxin-reduced preparations) may also be arranged upon request.

Is GPR158 relevant to neuropsychiatric disease research?

Yes. GPR158 is highly expressed in brain regions linked to mood regulation and stress response, and emerging studies associate it with conditions such as major depressive disorder and schizophrenia. This makes Recombinant Human GPR158 Protein a valuable tool for researchers investigating the molecular basis of these disorders and screening potential therapeutic targets.

References

1. Orlandi C, Bhatt DL, et al. GPR158: an orphan receptor with emerging roles in neuropsychiatric disease. Review — specific citation details require verification.
2. Aladdin Scientific. Recombinant Human GPR158 Protein, SKU rp368046, Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.