Protocols

Schiff's Reagent

· Reviewed · Published 9 jun 2025 · Updated 27 jul 2023 · 8 min read

Recipe

1.Dissolve 5g of basic fuchsin in 900ml of boiling distilled water

2.Cool to approximately 50oC and slowly add 100ml of 1N HCl

3.Cool to approximately 25oC and dissolve 10g of K2S2O5

4.Shake for 3 minutes and incubate in the dark at room temperature for 24 hours

5.Add 5 grams of fine activated charcoal and shake for 3 minutes

6.Filter solution (should be clear)

7.Store at 4oC in a foil covered bottle

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Aladdin Scientific. "Schiff's Reagent" Aladdin Knowledge Base, updated 27 jul 2023. https://www.aladdinsci.com/us_es/faqs/schiffs-reagent-en.html

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