Sucrose preference experiments
Sucrose preference experiments
The sucrose preference experiment is based on the fact that mice have a preference for sweetness, and mice are given a bottle of sucrose solution and a bottle of water to choose from to compare their behavioral preference for sucrose solution and water.
Operation method
Sucrose preference test (SPT)
Principle
The sucrose preference experiment is based on the fact that mice have a preference for sweetness, and mice are given a bottle of sucrose solution and a bottle of water to choose from to compare their behavioral preference for sucrose solution and water.
Materials and Instruments
One bottle of sucrose solution, one bottle of water. Move 1. keep the mice individually and acclimatize them to the environment for one week before performing the experiment. 2. Prepare sucrose solution with water: a. Fill a 50 ml centrifuge tube with about 40 ml of water and cap the pipette. b. Invert the tube so that the bubbles in the pipette rise. c, When inverting, make sure the water line is below the tapered end. d, Label each tube (e.g., "A" and "B" in permanent marker) and use the permanent marker to mark the location of the water line. e, Alternatively, the initial bottle weight can be recorded. f, Place the 2 inverted tubes in the metal lid of the cage and try not to shake to avoid dripping. g, Both bottles should be placed on one side of a separate metal rack with food on the other side. h, Make sure the rubber stopper is flush with the locking mechanism and the steel straw extends under the metal cage lid. i. Place the food on the other side of the wire lid. 3. Assess baseline water consumption daily for 4 consecutive days. To assess water consumption, weigh the bottle or mark the volume level. a. If making weight measurements, record the weight of the tube before and after consumption (1 g = 1 ml of water). b, For volume measurements, use a permanent marker to record the volume level on the tube. c, It is recommended that a different color marker be used each day. d, Do not invert the tube when marking the volume (pipette cap vertical). e, Monitor for any problems with liquid flow and look under the pipette for signs of spillage, e.g. wet bedding. f, Switch the position of the tubes daily (left to right). Indicate the mouse's preference for sucrose by calculating % = sugar-water consumption / ( sugar-water consumption + pure water consumption) × 100%. Image cited in Eagle, A. L., Mazei-Robison, M. and Robison, A. J. (2016). Sucrose Preference Test to Measure Stress-induced Anhedonia. bio-protocol 6(11): e1822. doi: 10.21769/BioProtoc.1822. Caveat 1. switch the position of sucrose solution and water every day to prevent inaccurate results in mice due to positional fixation. 2. Fix the experimental conditions throughout the experiment to prevent inaccurate results due to external conditions. Common Problems 1. In this experiment, the mice should not have too much bedding; the mice will pile up the bedding during nesting or when under stress, and the bedding touching the pipette will cause the pipette to lose its contents, making the volume of water or sucrose solution inaccurate. 2. How should the concentration of sucrose be determined? A: If the results of the experimenter's hypothesis indicate that preference may decrease, a higher concentration is recommended. However, if the experimenter anticipates an increase in preference, a lower concentration may be more appropriate. For more product details, please visit Aladdin Scientific website.