Biological processes are complex and determined by networks of interactions, called interactome networks, which include protein-protein interactions. The protein interactome is elemental to cellular function, but our full understanding of it is incomplete. One assay that has helped us begin to ...
Coelenterazine 400a is a chemically modified analog of native coelenterazine, designed to emit at shorter wavelengths (~395–400 nm) for high-sensitivity bioluminescent applications. This article explores its structural characteristics, origins, specific utility in BRET² and Ca²⁺ assays, ...
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