Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Aldehyde dehydrogenase (ALDH) is a class of oxidoreductases that use NAD+ or NADP+ as cofactors and mainly catalyze the oxidation of aldehyde substrates into corresponding carboxylic acids. Aldehydes can originate from ethanol metabolism, lipid peroxidation, sugar oxidation, drug metabolism, and ...
Lipase (Lipase, EC 3.1.1.3) belongs to the carboxylic ester hydrolase family and primarily catalyzes the stepwise hydrolysis of triacylglycerols (TAGs) to diacylglycerols (DAGs), monoacylglycerols (MAGs), glycerol, and free fatty acids (FFAs).
Acid phosphatase (Acid Phosphatase, ACP) refers to a family of hydrolases that catalyze the hydrolysis of phosphomonoester bonds under acidic conditions, dephosphorylating a broad range of phosphorylated substrates and releasing inorganic phosphate (Pi). ACP is widely distributed across animals, ...
Papain is one of the most representative cysteine proteases in the latex of unripe papaya fruit. With relatively mild reaction conditions, broad substrate tolerance, and good formulatability, it has long supported applications in food processing, industrial processing aids, biological sample ...
Enzyme activity reflects the rate capability by which an enzyme catalyzes the conversion of substrate to product, and is a key parameter for characterizing metabolic state, evaluating pathological changes, describing the intensity of biological processes, and optimizing industrial biocatalytic ...
Techniques often explored alongside immunological experiments.
