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HTC

Browse protocols, FAQs, guidelines, and technical articles tagged “HTC”, newest first. For research use only.

1 resources · protocols, FAQs, guidelines & articles
Article

Why Did Nickel Agarose Beads Lose Their Color?

The blue color of nickel agarose beads arises from coordination between Ni²⁺ and immobilized ligands (e.g., NTA/IDA/TED-type chelators). When loading His-tagged proteins, the local coordination environment changes and the color often becomes temporarily lighter; if strong chelators such as ...

Updated ·4 min read

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