In mammalian stable cell line generation, lentiviral/plasmid transduction selection, CRISPR functional screening, and multi-gene co-expression experiments, antibiotic selection systems are used to enrich cells that have successfully integrated or expressed resistance genes.
Hygromycin B is an aminoglycoside antibiotic. Enriched with highly polar functional groups such as hydroxyl and amino groups, it exhibits good water solubility and strong solvation capacity, and can interact with key structural domains of the ribosomal translation machinery, thereby inhibiting ...
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