Topic

Ligand

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

7 resources · protocols, FAQs & articles
article

Low-Pd Coupling of Aryl/Heteroaryl Chlorides (Ar–Cl): How to Make It Robust—Buchwald Ligand/Precatalyst Initiation Consistency and Scale-Up Reproducibility (with Controls and Selection Tables)

Under the combined constraints of “aryl/heteroaryl chlorides (Ar–Cl) + low Pd loading + scale-up reproducibility,” the most common source of instability does not necessarily show up as “a complicated mechanism,” but rather as a more straightforward engineering issue: whether the mode ...

Updated Jul 202612 min read✓ Reviewed
article

Low-Pd Coupling of Aryl/Heteroaryl Chlorides (Ar–Cl): How to Make It Robust—Buchwald Ligand/Precatalyst Initiation Consistency and Scale-Up Reproducibility (with Controls and Selection Tables)

Under the combined constraints of “aryl/heteroaryl chlorides (Ar–Cl) + low Pd loading + scale-up reproducibility,” the most common source of instability does not necessarily show up as “a complicated mechanism,” but rather as a more straightforward engineering issue: whether the mode ...

Updated Feb 202612 min read✓ Reviewed
article

Make “Aryl Chlorides + Low Pd Loading + Scale-Up Reproducibility” Reliable: The Initiation and Durability Logic of Pd–NHC (Palladium–N-Heterocyclic Carbene) Cross-Coupling (with Selection Navigation and Product Tables)

Modern synthesis increasingly faces the same hard requirement set: use less Pd, deal with “less reactive” substrates (typified by aryl chlorides), and still maintain yield, impurity profile, and batch-to-batch reproducibility upon scale-up. When the substrate is harder to activate (Ar–Cl), ...

Updated Feb 202612 min read✓ Reviewed
article

Nicewicz Photoredox Catalysts for Anti-Markovnikov Alkene Hydrofunctionalization

Although the Markovian rule plays a key role in explaining the mechanism of olefin Addition reaction, and it has also been widely used in scientific research and practical production, there are very few kinds of addition products with anti Markovian rules obtained through catalytic processes.

Updated Oct 202312 min read✓ Reviewed
article

BINOL and Derivatives

BINOL and its derivatives are one of the most widely used types of ligands in asymmetric synthesis; being utilized in a variety of reactions including——Diels-Alder, carbonyl addition and reduction, Michael addition, and a variety of other reactions. Although great success has been achieved ...

Updated Oct 202312 min read✓ Reviewed
article

N-Heterocyclic Carbene (NHC) Ligands

With rapid advances in the field of cross-coupling reactions of unactivated substrates catalyzed by metal complexes, the chemical market has been revolutionized by the introduction of a large library of achiral and chiral phosphine ligand compounds. Phosphine ligands have shown positive effects ...

Updated Oct 202312 min read✓ Reviewed

Related topics

Techniques often explored alongside immunological experiments.

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