Dissolve 35 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). If precipitate forms on autoclaving, medium may be filter-sterilized using a 0.2 micron filter.
Component
g/liter
Dextrose
2.5
Potassium Phosphate Dibasic
2.5
Sodium Chloride
5
Soytone
3
Tryptone
17
Yeast Extract
5
Total: 35g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.
Homogeneous: Proprietary blending technology ensures complete mixing of components.
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