Laboratory Protocols & SOPs Library

Tris-Buffered Saline (TBS)

Recipe

1.Dissolve the following in 800ml of distilled H2O.

  • 8g of NaCl, 0.2g of KCl

  • 3g of Tris base

2.Add 0.015g of phenol red.

3.Adjust the pH to 7.4 with HCl.

4.Add distilled H2O to 1L.

5.Sterilize by autoclaving.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "Tris-Buffered Saline (TBS)" Aladdin Knowledge Base, updated 9 jun 2025. https://www.aladdinsci.com/us_es/faqs/tris-buffered-saline-tbs-en.html
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