Urine specimen inoculation experiment
Urine specimen inoculation experiment
Urine specimen inoculation tests can be used to: check for the presence of Gram-negative or positive bacteria in urine in the clinic, which can be reported.
Operation method
Urine specimen inoculation experiment
Principle
Urinary tract infection is a relatively common disease, is a large number of microorganisms in the urethra growth and reproduction caused by inflammatory lesions, especially in women and children are more common, the clinic often use urine bacterial culture identification and drug sensitivity testing for the diagnosis of urinary tract infection, but the treatment time is long, not conducive to the patient to receive timely treatment. The direct urine bacterial smear microscopy test time is short and simple and feasible, the results are rapid and accurate, according to the morphology of the bacteria, staining and other pathogenic bacteria to make the most preliminary judgment on the type of urinary tract infection also has a high diagnostic rate, the clinical treatment of medication has also achieved remarkable results.
Materials and Instruments
Patient urine Move 1、Smear examination: general bacterial smear: take 5-10 ml of urine into a sterile tube by aseptic operation, centrifuge it at 3000 r/min for 30 min, pour off the supernatant, take the sediment, smear it, stain it with Gram stain and examine it by microscope. If gram-negative or positive bacteria are found, a report can be made. Caveat Urine specimens were subjected to direct smear microscopic examination of urine bacteria and were compared and analyzed with urine bacterial culture identification and drug sensitivity results. Common Problems The results of direct urine bacterial smear microscopy are reported rapidly, which can quickly and accurately guide the clinical treatment and medication, and this method can be widely used in the clinic. For more product details, please visit Aladdin Scientific website.
Blood plate Culture medium Gram stain
Slides
2. Urine bacterial culture: clinically, mid-range urine is mostly used for bacterial culture, and colony counting should be done for the culture results to have diagnostic value.
(1) Plate inoculation method: take 5 ul of urine with quantitative liquidizer, drip it on the plate culture medium, smear it with inoculation ring, incubate it at 35 ℃ for 18-24 hours, and multiply the number of growing colonies by 200 to find out the number of bacteria per ml.
(2) Media selection: Blood plate and Chinese orchid/McConkey are the basic media on which general Gram-positive and negative bacteria can grow.
3. Reporting method: In 10% of patients with urinary tract infection, two bacteria may be isolated from the same specimen and both are pathogenic. If more than 3 different species of microorganisms are detected in the same specimen, it should be considered that the specimen has been improperly collected or handled to be contaminated. In general, it is considered that: Gram-negative bacillus colony counts of urine specimens are greater than 105 CFU/ml, and gram-positive coccobacilli counts of more than 104 CFU/ml have diagnostic significance, and the results of the identification and drug sensitivity tests should be reported.