Formamide RNA Loading Buffer (10×) - BioReagent,Suitable for molecular biology,for NA electrophoresis,10×

Cat. No.: F1518788

GRADE & PURITY Suitable for molecular biology, BioReagent, for NA electrophoresis, 10×

Synonyms

10× RNA Loading Buffer | 10× RNA Loading Dye | 10× RNA Sample Loading Buffer | 10× RNA Gel Loading Buffer

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

Nucleic Acid Electrophoresis

Descripción general

Formamide Loading Buffer (10×) is a 10-fold concentrated loading buffer specifically designed for RNA electrophoresis. It is primarily composed of deionized formamide, bromophenol blue, EDTA, and other components, with bromophenol blue serving as the electrophoresis tracking dye. When diluted to 1×, the buffer has a relatively high density, allowing samples to quickly settle into the wells, resulting in clear and easily observable electrophoretic band migration. For use, simply mix the sample with the buffer; no further dilution is required. For research use only. Not for use in diagnostic procedures or other purposes. 

Procedure (For Reference Only)

1. Mix the RNA sample with Formamide Loading Buffer (10×) at a ratio of 1:9.

2. Directly load the mixture into the wells of a denaturing RNA gel for electrophoresis.

Notes

1. If the single-use volume is small, it is recommended to aliquot the reagent before use to reduce the risk of contamination.

2. Strictly avoid RNase contamination throughout the entire experimental procedure.

3. Please wear laboratory appropriate attire and disposable gloves during operation to ensure experimental safety.

4. Use the reagent as soon as possible after opening and keep it sealed to avoid affecting experimental results.

Specifications

Sinónimos

10× RNA Loading Buffer | 10× RNA Loading Dye | 10× RNA Sample Loading Buffer | 10× RNA Gel Loading Buffer

Especificaciones y pureza

BioReagent, Suitable for molecular biology, for NA electrophoresis, 10×

Estabilidad y almacenamiento

Store at 2-8℃ long term (12 months). Store in the dark.

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C, Protected from light

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, for NA electrophoresis, Suitable for molecular biology

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive