≥90%(HPCE), 10000 x in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
AIE-Gelgreen Nucleic Acid Gel Dyes is a tetraphenylethylene derivative based on aggregation-induced emission (AIE) fluorescent materials, with typical AIE properties. This product can be used as a staining agent for various nucleic acid electrophoresis, suitable for staining nucleic acid fragments of different sizes. It is compatible with standard gel imaging systems and gel observation devices excited by visible light, especially those excited by blue visible light.
AIE-Gelgreen Nucleic Acid Gel Dyes is a highly sensitive, stable, versatile and highly compatible nucleic acid gel dye, which can be used to stain dsDNA, ssDNA and RNA in agarose gels and polyacrylamide gels. It exhibits extremely high sensitivity, with a detection limit as low as the pg level. In terms of gel staining, it is an ideal alternative to silver staining. Clear and bright bands can be obtained within 5 minutes, with simple operation and intuitive results. Moreover, AIE-Gelgreen nucleic acid gel dye has a wide excitation spectrum, thus featuring strong compatibility. It can replace ethidium bromide (EB) and other gel dyes without changing any imaging system, and is suitable for gel imaging and detection systems that originally use SYBR Green or SYBR Gold as nucleic acid dyes, making it an ideal choice for use with laser scanners. In addition, it allows nucleic acid detection using blue light lamp or blue light imagers that are harmless to the human body, thereby avoiding the mutagenicity of conventional ultraviolet detection to nucleic acid samples and the damage of ultraviolet rays to human eyes and skin.
Product Advantages
(1) High sensitivity: Suitable for staining nucleic acid electrophoresis of fragments of various sizes, with a sensitivity as high as the pg level;
(2) Strong stability: Can be heated in a microwave oven, and stored at room temperature for 2 years without affecting its effectiveness;
(3) Wide compatibility: The channel is compatible with standard gel imaging systems and gel observation devices excited by visible light;
(4) Fast and simple: Clear and bright bands can be obtained within 5 minutes of soaking staining, without the need for decolorization or rinsing;
(5) Strong operability: It has a high signal-to-noise ratio within the concentration range of 0.1× to 1×;
(6) Wide applicability: Suitable for agarose gel or polyacrylamide gel electrophoresis, and can stain dsDNA, ssDNA or RNA;
Experimental Methods
I. Gel staining method (pre-staining method) (used the same as EB, recommended)
1.Prepare agarose gel according to routine operations, add concentrated AIE-Gelgreen Nucleic Acid Gel Dyes to make its final concentration in the gel 1× (for example, to prepare 100mL gel, add 10μL of dye; the dosage can be adjusted according to actual conditions), shake gently, and pour the gel.
2.Perform electrophoresis according to routine methods and observe the results.
II. Spot staining method
1.Prepare agarose gel according to routine operations. After the gel solidifies, mix the dye with the sample and loading buffer, then load it into the sample well (does not affect the migration rate).
2.Perform electrophoresis according to routine methods and observe the results.
III. Soaking staining method (post-staining method)
1.Perform electrophoresis according to routine methods.
2.Dilute the AIE-Gelgreen Nucleic Acid Gel Dyes concentrate about 2500 times with ddH2O into 0.1M NaCl to prepare 2× staining solution. (For example, add 15-20μL of 10000× AIE-Gelgreen Nucleic Acid Gel Dyes concentrate and 5mL of 1M NaCl to 45mL of ddH2O).
3.Carefully put the gel into a suitable container, slowly add enough 2× staining solution to submerge the gel. Stain with shaking at room temperature for about 5 minutes. The optimal staining time varies slightly depending on the gel thickness and agarose concentration. For 3.5-10% acrylamide gels, the staining time can be appropriately extended. Then observe the results.
Precautions
1.Please mix briefly before use;
2.For your safety and health, please wear a lab coat and disposable gloves during operation;
3.This product is only for scientific research purposes.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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