GlcNAc-1-P uridyltransferase (PmGlmU)

Cat. No.: G1445241

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), ≥5 U/mg protein; Protein concentration: See COA; expressed in E.coli

Accession #

Q9CK29

Protein Tag

C-His

Expression system

E. coli

Bioactivity

≥5 U/mg protein

Descripción general

N-Acetylglucosamine 1-phosphate uridyltransferase (PmGlmU), EC 2.3.1.157, derived from Pasteurella multocida. This enzyme is used to catalyze the formation of UDP‑GlcNAc from GlcNAc‑1‑P, working in conjunction with PmPPA and NahK to activate GlcNAc. The product is recombinantly expressed in E. coli and exhibits high activity.

Reaction

GlcNAc‑1‑P + UTP → UDP‑GlcNAc + PPi

Protocol

Add substrates to the reaction system according to the desired composition. Include 5 mM MgCl₂ and, if desired, a buffer salt such as Tris‑HCl. Adjust the pH to 7.5–8.5, then add an appropriate amount of PmGlmU (depending on the reaction requirements).

Mix thoroughly and incubate the sample at 37 °C for 30–60 min. The recommended reaction buffer is Tris‑HCl (pH 8.0), but other common biological buffers such as Tris or PBS are also suitable.

Note: When scaling up the system, the incubation time or enzyme amount should be adjusted according to actual conditions; low‑speed shaking may be used to ensure proper mixing.

Precautions

For your safety and health, please wear a lab coat and disposable gloves when handling this product. This product is for research use only!

Specifications

Product Name

GlcNAc-1-P uridyltransferase (PmGlmU)

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Especificaciones y pureza

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), ≥5 U/mg protein; Protein concentration: See COA; expressed in E.coli

Bioactividad

≥5 U/mg protein

Número de adhesión

Q9CK29

Peso molecular previsto

57 kDa

Número de la Comisión de Enzimas

EC 2.3.1.157

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

≥5 U/mg protein; Protein concentration: See COA; expressed in E.coli

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definición de unidad

The amount of enzyme required to catalyze the formation of 1 μmol UDP-GlcNAc from GlcNAc-1-P and UTP per minute at 37°C