β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

CAS: 9001-45-0 Cat. No.: G755243 Número EC: 232-606-8

GRADE & PURITY Type H-3, aqueous solution,≥90,000 units/mL

Accession #

P09811

Descripción general

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application:

β-Glucuronidase from Helix pomatia has been used:

• to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detection
• as a component in the protoplasting solution
• for the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)
• to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples

β-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Specifications

Product Name

β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

Especificaciones y pureza

Type H-3, aqueous solution, ≥90, 000 units/mL

Mecanismos bioquímicos y fisiológicos

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.Used for the hydrolysis of glucuronide co

Número de adhesión

P09811

CAS

9001-45-0

Número de la Comisión de Enzimas

3.2.1.31(BRENDA, IUBMB)

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

Type H-3, aqueous solution,≥90,000 units/mL

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Referencias

1. Bairen Du, Jingjing Zhang, Yanjie Dong, Junwei Wang, Longwen Lei, Rengfei Shi.  (2020)  Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis.  STEROIDS,      [PMID:32603755] [10.1016/j.steroids.2020.108691]

2. Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li, Yongning Wu.  (2018)  Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection.  TALANTA,      [PMID:30172485] [10.1016/j.talanta.2018.07.073]