Type H-5, lyophilized powder,≥400,000 units/g solid for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application:
β-Glucuronidase from Helix pomatia has been used:
in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)
β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.
New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Specifications
Product Name
β-Glucuronidase from Helix pomatia, CAS No.9001-45-0
Especificaciones y pureza
Type H-5, lyophilized powder, ≥400, 000 units/g solid
Mecanismos bioquímicos y fisiológicos
β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.Used for the hydrolysis of glucuronide co
1.Bairen Du, Jingjing Zhang, Yanjie Dong, Junwei Wang, Longwen Lei, Rengfei Shi. (2020) Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis. STEROIDS, [PMID:32603755][10.1016/j.steroids.2020.108691]
2.Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li, Yongning Wu. (2018) Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection. TALANTA, [PMID:30172485][10.1016/j.talanta.2018.07.073]
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