Goat Anti-Human IgG Fc (AF594) - Secondary antibody, specific to Human IgG Fc

Cat. No.: Ab216755

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Human

Isotype

Goat IgG

Conjugation

AF594

Descripción general

Goat Anti-Human IgG Fc Antibody, Secondary antibody, could be used for Flow Cytometry, IF/ICC and so on.

Specifications

Product Name

Goat Anti-Human IgG Fc (AF594) - Secondary antibody, specific to Human IgG Fc

Sinónimos

Goat Anti-Human IgG Fc Antibody | Goat Anti Human IgG Fc Antibody | Anti-Human IgG Fc Antibody | Anti Human IgG Fc Antibody

Especificaciones y pureza

ExactAb™, Validated, 1.0 mg/mL

Especies hospedadoras

Goat

Especificidad

Human IgG Fc

Inmunógeno

Human IgG Fc

Conjugación

AF594

Excitación (nm)

Yellow-Green Laser(594nm)

Emisión (nm)

618nm

Ex/Em(nm)

Ex:590nm;Em:618nm

Grado

ExactAb™, Validated

Método de purificación

antigen affinity chromatography

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Imágenes

IF/ICC: Goat Anti-Human IgG Fc (AF594) (Ab216755)

Goat Anti-Human IgG Fc (AF594) (Ab216755) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG Fc (AF594) (Ab216755) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG Fc (AF594) (Ab216755)

Goat Anti-Human IgG Fc (AF594) (Ab216755) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Goat Anti-Human IgG Fc (AF594) (Ab216755) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  1/100-1/1000 ;

• IF/ICC:

  1/100-1/1000 ;