Goat Anti-Human IgG (LumiDye™ Violet 421) - Secondary antibody

Cat. No.: Ab220501

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Human

Isotype

Goat IgG

Conjugation

172

Descripción general

Purified goat polyclonal antibody, suitable for Flow Cytometry and IF/ICC.

Specifications

Product Name

Goat Anti-Human IgG (LumiDye™ Violet 421) - Secondary antibody

Sinónimos

Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I

Especificaciones y pureza

ExactAb™, Validated, 1.0 mg/mL

Especies hospedadoras

Goat

Conjugación

172

Excitación (nm)

Violet Laser(405nm)

Emisión (nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grado

ExactAb™, Validated

Método de purificación

antigen affinity chromatography

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Imágenes

IF/ICC : Goat Anti-Human IgG (LumiDye™ Violet 421) (Ab220501)

Goat Anti-Human IgG (LumiDye™ Violet 421) (Ab220501) - IF/ICC
IF analysis of EpCAM (blue) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (LumiDye™ Violet 421) (Ab220501) at a dilution of 1/1000 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  1:100-1:2000 ;

• IF/ICC:

  1/100-1/1000 ;