GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
ExactAb™, Validated, See COA ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.
Specifications
Product Name
Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) - Secondary antibody, specific to Rabbit IgG
This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.
Imágenes
Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) (Ab220047) - IF/ICC IF analysis of HA tag (green) in HEK293T cells transfected with a mCherry-HA tag. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HA tag Antibody (Ab106771) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) (Ab220047) at 2mL/well for 1 hour at room temperature in the dark (green). A 6-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Aplicación
Aplicación
Dilution info
IF/ICC
Use at an assay dependent volume.
Flow Cytometry
100μL/test
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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