Carmine is a commonly used basic dye, which is often applied for nuclear staining, as well as chromosome fixation and staining. When observing plant cell mitosis, alkaline staining solutions are required for chromosome staining, and acetocarmine or gentian violet staining solution can be used at this time. In pollen detection, acetocarmine can be adopted to determine whether pollen is in the uninucleate stage.
Aladdin Grignard Ammonium Alum Magenta Stain Solution is mainly composed of high-concentration ammonium aluminum sulfate and carmine (also known as cochineal). It is acidic in pH, and is primarily used for cytological staining of samples such as pollen, animal and plant tissue sections, with low risk of over-staining. This reagent is for research use only and not suitable for clinical diagnosis or other purposes.
Materials to Be Prepared by the User:
Glass slides, coverslips, optical microscope
Operating Procedures (For Reference Only):
1. Pollen grains: Place a small amount of pollen on a glass slide, add an appropriate volume of ferric alum acetocarmine staining solution, mix thoroughly, cover with a coverslip immediately and stain for 15–60 minutes. Then absorb the excess liquid and observe under a microscope.
2. Animal and plant tissue sections: Perform routine treatment (e.g., deparaffinize to water), add Gregory’s alum carmine staining solution dropwise and stain for 10–30 minutes. Rinse with tap water, mount the slide, and observe under a microscope.
3. Some fresh plant tissues (e.g., onion epidermis): Immerse the tissue directly in Gregory’s alum carmine staining solution for 10–30 minutes, rinse with tap water for 2–3 minutes, place on a glass slide, cover with a coverslip, and observe under a microscope.
4. If the staining effect is unsatisfactory: Gently and quickly pass the slide back and forth over an alcohol lamp several times to disrupt the chromatin and enhance nuclear staining.
Precautions:
1. Observe the sample under a microscope immediately after staining.
2. Since this reagent contains high-concentration weak acid and has an irritating odor, please take appropriate personal protective measures.
3. Store the reagent in a sealed container; otherwise, the staining efficiency will decrease.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
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