High ROX Reference Dye (50×) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,50×

Cat. No.: FP1509095

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 50×

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, qPCR

Descripción general

High ROX Reference Dye (50×) is an inert fluorescent dye used in real-time quantitative PCR (qPCR) or RT-PCR to correct for well-to-well variations in fluorescence signals. It does not participate in the PCR reaction, and its fluorescence signal remains stable throughout the process. ROX dye can correct for fluorescence signal variations caused by factors such as uneven illumination, optical differences between wells (e.g., droplets, bubbles), and variations in reaction system composition. 

Instruments requiring High ROX Reference Dye (50×):

ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus.

Instruments requiring Low ROX Reference Dye (50×):

ABI 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex.

Stratagene MX3000P, MX3005P, MX4000P.

Instruments not requiring ROX correction:

Bio-Rad CFX96, CFX384, iCycler iQ, iQ 5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4.

Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96.

Eppendorf Mastercycler ep realplex, realplex 2 s.

Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000.

Thermo Scientific PikoReal Cycler.

Cepheid SmartCycler.

Illumina Eco qPCR.

Instructions for Use

Preparation of qPCR system using High ROX Reference Dye (50×):

For a 20 µL qPCR reaction system, add 0.4 µL of High ROX Reference Dye (50×).

Note:

• The dye can be added proportionally according to the total reaction volume.

• When preparing a multi-well system, it is recommended to prepare a master mix, ensuring thorough mixing before use.

Precautions

1. After receiving the reagent, it can be aliquoted for use to avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Especificaciones y pureza

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 50×

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot. Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C, Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive