Hyaluronidase, CAS No.37326-33-3

CAS: 37326-33-3 Cat. No.: H754463 Número EC: 253-464-3

GRADE & PURITY Bioactive, ActiBioPure™, Native, Endotoxin tested, High Performance, EnzymoPure™, ≥1000 IU/mg，derived from bovine testicles

Accession #

Q5E985,Q8SQG8

Endotoxin Concentration

<0.2 EU/IU

Bioactivity

≥1000 IU/mg enzyme powder

Descripción general

Hyaluronidase is widely present in nature.In Hyaluronidase is widely present in nature.In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards. 

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue；as a component of digestion solution for the derivation of germline stem cells from testicular tissue.

Specifications

Product Name

Hyaluronidase, CAS No.37326-33-3

Sinónimos

Hyaluronate Lyase | hyaluronoglucosaminidase | chondroitinase | chondroitinase I | hyaluronidase | hyaluronoglucosidase | Hyaluronate 4-glycanohydrolase | HYAL1 | HYAL2

Grado

ActiBioPure™, Bioactive, Endotoxin tested, High Performance, Native, EnzymoPure™

Especificaciones y pureza

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, endotoxin tested, ≥1000 IU/mg，derived from bovine testicles

Mecanismos bioquímicos y fisiológicos

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Bioactividad

≥1000 IU/mg enzyme powder

Concentración de endotoxinas

<0.2 EU/IU

Número de adhesión

Q5E985, Q8SQG8

CAS

37326-33-3

Número de la Comisión de Enzimas

3.2.1.35

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

≥1000 IU/mg，derived from bovine testicles

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definición de unidad

Under conditions of 37 ℃ and pH 5.3-5.7, one unit can cause a 0.330% change in transmittance of 2.0 mL reaction mixture at 600 nm (detected at 45 min)

Referencias

1. Yidan Chen, Tiantian Feng, Xiaohong Zhu, Yuting Tang, Yao Xiao, Xiuhua Zhang, Sheng-Fu Wang, Dong Wang, Wei Wen, Jichao Liang, Huayu Xiong.  (2024)  Ambient Synthesis of Porphyrin-Based Fe-Covalent Organic Frameworks for Efficient Infected Skin Wound Healing.  BIOMACROMOLECULES,      [PMID:38720431] [10.1021/acs.biomac.4c00261]

2. Mengyao Qi, Yi Ke, Yifei Li, Peng Li, Huakuo Zhou, Xia Zhang, Jieshi Chen, Jun Meng.  (2024)  Au@Pt nanozyme-based smart hydrogel for visual detection of hyaluronic acid.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.137144]

3. Tao Meng, Zhengyu Zhao, Mengqiang Song, Rui Wang, Xu Xu, Min Yang, Weihua Li, Shiyong Song.  (2025)  Carboxybetaine modified chitosan as viscosupplementation for osteoarthritis therapy.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40090277] [10.1016/j.ijbiomac.2025.142155]

4. Yu Pang, Jie Lv, Chengcai He, Chengda Ju, Yulong Lin, Cong Zhang, Meng Li.  (2024)  Covalent organic frameworks-derived carbon nanospheres based nanoplatform for tumor specific synergistic therapy via oxidative stress amplification and calcium overload.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:38330663] [10.1016/j.jcis.2024.01.217]

5. Shiyi Yao, Hui Yuan, Luqi Yang, Yin Zhang, Hanyu Wang, Renxuan Li, Tingjun Ye, Wenguo Cui, Lei Wang.  (2025)  Downregulation of the PI3K/AKT/mTOR/MMP-13 pathway for promoting interface healing via lubricating microspheres.  Acta Biomaterialia,      [PMID:39761787] [10.1016/j.actbio.2025.01.001]

6. Jing Zhang, Yunfeng Zeng, Yongyuan Heng, Yu Shen, Ximeng Sun, Yijia Wang, Haiping Zheng, Ming Zeng, Ziyi Yu.  (2024)  Enzyme-Assisted Activation Technique for Producing Versatile Hydrogel Microparticle Scaffolds with High Surface Chemical Reactivity.  ADVANCED FUNCTIONAL MATERIALS,      [PMID:] [10.1002/adfm.202400858]

7. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li.  (2024)  Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound.  ACS Nano,      [PMID:38949962] [10.1021/acsnano.4c02921]

8. Huiping Yang, Yinning Lin, Qian Mo, Zhaoquan Li, Fan Yang, Xinchun Li.  (2024)  Monitoring Enzymatic Reaction Kinetics and Activity Assays in Confined Nanospace.  ANALYTICAL CHEMISTRY,      [PMID:39024010] [10.1021/acs.analchem.4c01901]

9. Shuo Yu, Lu Zhang, Yanshen Yang, Meijuan Wang, Tingting Liu, Wenwen Ji, Yang Liu, Hao Lv, Yang Zhao, Xi Chen, Tinghua Hu.  (2024)  Polydopamine-Based Resveratrol-Hyaluronidase Nanomedicine Inhibited Pancreatic Cancer Cell Invasive Phenotype in Hyaluronic Acid Enrichment Tumor Sphere Model.  ACS Pharmacology & Translational Science,      [PMID:38633596] [10.1021/acsptsci.3c00304]

10. Xin Jin, Chengxiong Wei, Kai Li, Peinan Yin, Chengwei Wu, Wei Zhang.  (2024)  Polyphenol-mediated hyaluronic acid/tannic acid hydrogel with short gelation time and high adhesion strength for accelerating wound healing.  CARBOHYDRATE POLYMERS,      [PMID:39048222] [10.1016/j.carbpol.2024.122372]

11. Dandan Wei, Guanhua Jiao, Yinghua Tao, Liuxin Yang, Tao Liu, Fengya Jing, Tianzhu Zhang.  (2025)  Polypropylene Mesh Coated with Dual Cross-Linked Hyaluronic Acid/Polyvinyl Alcohol Composite Hydrogel with Antiadhesion and Angiogenesis Properties for Abdominal Wall Repair.  Advanced Materials Technologies,      [PMID:] [10.1002/admt.202401786]

12. Dandan Wei, Yulin Huang, Min Liang, Liuxin Yang, Guanhua Jiao, Yinghua Tao, Li Xu, Tianzhu Zhang, Zhenling Ji.  (2024)  Polypropylene mesh coated with hyaluronic acid/polyvinyl alcohol composite hydrogel for preventing bowel adhesion.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:38705326] [10.1016/j.ijbiomac.2024.132061]

13. Chao-fan He, Tian-hong Qiao, Xu-chao Ren, Mingjun Xie, Qing Gao, Chao-qi Xie, Peng Wang, Yuan Sun, Huayong Yang, Yong He.  (2025)  Printability in Multi-material Projection-Based 3-Dimensional Bioprinting.  Research,      [PMID:40041038] [10.34133/research.0613]

14. Zhu Dan, Zhong Hanyan, Shi Jingzhan, Liu Qiang, Wang Yiping.  (2025)  Ratiometric surface-enhanced Raman scattering quantification of extracellular matrix metalloproteinase-2 activity for tumor diagnosis.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,      [PMID:39966175] [10.1007/s00216-025-05792-5]

15. Yingying Chen, Dan Zhu, Hanyan Zhong, Zhixing Gan, Shenfei Zong, Zhuyuan Wang, Yiping Cui, Yiping Wang.  (2024)  Ultrasensitive Detection of Matrix Metalloproteinase 2 Activity Using a Ratiometric Surface-Enhanced Raman Scattering Nanosensor with a Core–Satellite Structure.  ACS Applied Materials & Interfaces,      [PMID:38204415] [10.1021/acsami.3c15344]

16. Shangting Du, Lingyu Li, Junhao Kou, Tianyi Zhu, Zhenyi Song, Yonghua Zhan, Daocheng Wu, Wenhua Zhan.  (2025)  Blackberry-Like Doxorubicin Loaded Hyaluronic Acid/Zinc Phthalocyanine Loaded Mesoporous Silica Nanocomposites for Long-Term Tumor Photodynamic and Chemotherapy Synergistic Therapy.  International Journal of Nanomedicine,      [PMID:40692537] [10.2147/IJN.S530041]

17. Jiancong Ni, Xiaochun Xu, Bifang Yang, Liyang Liu, Weiqiang Yang, Xiaoping Chen, Guiping Chen, Qingxiang Wang, Zhenyu Lin.  (2025)  Indicator-Free Detection of Hyaluronidase Based on a Conductivity-Regulated Bipolar Electrochemiluminescence Platform.  ANALYTICAL CHEMISTRY,      [PMID:40827572] [10.1021/acs.analchem.5c03409]

18. Haojie Qiu, Jingyan Zhu, Kaikai Zhang, Xinnan Wang, Yaoting Chu, Feng Zhang, Xuefeng Yang.  (2025)  Dual Drug-Loaded Hyaluronate-Based Self-Adaptable Hydrogel for Diabetic Infected Wound Healing.  BIOMACROMOLECULES,      [PMID:40845349] [10.1021/acs.biomac.5c00712]

19. Siying Huang, Simeng Xiao, Xuehao Li, Ranran Tao, Zhangwei Yang, Ziwei Gao, Junjie Hu, Yan Meng, Guohua Zheng, Xinyan Chen.  (2024)  Development of Dual-Targeted Mixed Micelles Loaded with Celastrol and Evaluation on Triple-Negative Breast Cancer Therapy.  Pharmaceutics,  16  (9): (1174).  [PMID:39339211] [10.3390/pharmaceutics16091174]

20. Wenjun Xue, Xiaoyu Kuang, Xuanzhen Meng, Boyu Sun, Zijun Zhao, Guizhao Liang.  (2025)  Identification and inhibition mechanism of novel collagen-derived hyaluronidase inhibitory peptides.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.106263]