GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
ExactAb™,Validated,5 μL/test ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human/Mouse/Rat ICAM1/CD54 Antibody, Monoclonal (15.2), could be used for Flow Cytometry.
Specifications
Product Name
ICAM1/CD54 Mouse mAb (AF488) - Primary antibody, specific to ICAM1/CD54
Especificaciones y pureza
ExactAb™, Validated, 5 μL/test
Mecanismos bioquímicos y fisiológicos
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In cas
Especies hospedadoras
Mouse
Especificidad
ICAM1/CD54
Inmunógeno
Human infant thymocytes and Sezary cells
Control positivo
Flow Cytometry: human peripheral blood monocytes
Conjugación
AF488
Excitación (nm)
Blue Laser(488nm)
Emisión (nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grado
ExactAb™, Validated
Concentración
5 μL/test
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes
ICAM1/CD54 Mouse mAb (AF488) (Ab306570) - Flow Cytometry
Flow cytometry analysis of ICAM1/CD54 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with ICAM1/CD54 Mouse mAb (AF488) (Ab306570) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).
ICAM1/CD54 Mouse mAb (AF488) (Ab306570) - Flow Cytometry
Flow cytometry analysis of ICAM1/CD54 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with ICAM1/CD54 Mouse mAb (AF488) (Ab306570) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245).
Aplicación
Aplicación
Dilution info
Flow Cytometry
5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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