GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Low Endotoxin?Low-endotoxin grade — endotoxin reduced to low controlled levels. Use in sensitive biological work where high endotoxin would interfere.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE&HPLC)See COA
Mouse anti Human/Mouse/Rat ICAM1/CD54 Antibody, Monoclonal (15.2), could be used for IHC, Flow, IP, WB and so on. Application Flow: Use at an assay dependent concentration. IHC: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Protein Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Specifications
Product Name
ICAM1/CD54 Mouse mAb - Primary antibody, specific to ICAM1/CD54, Mouse IgG1
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
ICAM1/CD54 Mouse mAb (Ab109231) - Flow Cytometry Flow Cytometry analysis of Ramos cells labelling ICAM1/CD54 (red) with ICAM1/CD54 Mouse mAb (Ab109231) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
ICAM1/CD54 Mouse mAb (Ab109231) - Flow Cytometry Flow Cytometry analysis of Raji cells labelling ICAM1/CD54 (red) with ICAM1/CD54 Mouse mAb (Ab109231) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC
Use at an assay dependent concentration.
Flow Cytometry
Use at an assay dependent concentration.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Dilution Calculator
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Reconstitution Calculator
Reseñas
Reseñas de cliente
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