CALIDAD & PUREZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
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Almacenamiento y envío
Store at -20°C,Avoid repeated freezing and thawing Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Documentos de calidad
SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.
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Prueba documental
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Descripción general
Mouse anti Human IL-6 Antibody, clone C08/5B10, could be used for a Capture Antibody in sandwich ELISAs
Especificaciones
Product Name
IL-6 Mouse mAb - Primary antibody, specific to Human IL-6
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induc
Especies hospedadoras
Mouse
Especificidad
Human IL-6
Inmunógeno
Recombinant protein corresponding to Human IL-6
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Pares emparejados
[Ab220858] IL-6 Mouse mAb as Detection Antibody in ELISA
Concentración
1.0 mg/mL
Product Line
Productos biológicos
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Sandwich ELISA - IL-6 standard curve Sandwich ELISA detection of Recombinant Human IL-6 Protein (rp156005) using IL-6 Mouse mAb (Ab220856) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-6 Mouse mAb (Ab220858) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Detection range: 0.03 ng/mL - 2000.0 ng/mL
Indirect ELISA - IL-6 standard curve Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 µg/mL overnight at 4℃. Coated protein probed with IL-6 Mouse mAb (Ab220856). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody. EC₅₀: 29.72 ng/mL
Aplicación
Aplicación
Dilution info
ELISA
1.0 µg/mL as a Detection antibody in ELISA
Documentación
📋 Hoja de datos de seguridad (SDS)
Documento completo sobre peligros, manipulación, almacenamiento y cumplimiento normativo.
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Dilution Calculator
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Reconstitution Calculator
Reseñas
Reseñas de cliente
Preguntas frecuentes
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IL-6 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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