Immobilized Penicillin G acylase, CAS No.9014-06-6

CAS: 9014-06-6 Cat. No.: A1435677 Número EC: 232-753-8

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥500 U/g wet weight

Accession #

P06875

Bioactivity

≥500 U/g wet weight

Descripción general

  It produces 6-APA (6-aminopenicillanic acid) from Penicillin G.

Standard

Activity	≥200 U/g wet weight
Size of immobilization support	150~300 μm (> 95%)
Water content	< 60%
Enzyme assay	One unit (U) represents the amount of enzyme required for adding 1μmol NaOH per min to adjust pH with degradation of Pen G to 6-APA in 50mM K-phosphate, pH 8.0, at 28℃.

Application

6-APA production from Penicillin G.

Characteristics

• Very high specific activity
• High substrate selectivity
• High stability (reusable more than 800 cycles)
• High production rate of 6-APA (more than 98.5%)

Reaction condition

pH	8.0 ± 0.2
Temperature	28~29℃
Solvent	Phosphate buffer or water
Enzyme amount	7~15 U/ml
Reaction time	2~3 hours

Scheme for enzyme reaction

Production instruction

Category	Contents
Materials	- Catabolic enzyme: Immobilized Penicillin G acylase (200U/g wet, 60% water content) - Pen-G (penicillin G potassium) - Water - 3M ammonium hydroxide - HPLC - Chromatography column (Hypersil ODS, 5μm, 125mm*4.6mm)
Condition	- pH: 7.9~8.0 - Temperature: 28℃ - CPC -Na: 215mM - Enzyme: 7~15 U/ml (with 130ml) - Reaction solution: Water
Step	1. Remove the water after washing immobilized enzyme with water more than five times. 2. Dissolve 215mM Pen G in 130ml water. 3. Leave substrate in reactor at 28℃ for 10 minutes. 4. Pour the catabolic enzyme(Immobilized Penicillin G acylase 7~15 U/ml) in conversion reactor and measure time simultaneously. 5. Adjust pH to 8.0 with 3M ammonium hydroxide. 6. Extract 0.1ml reaction solution(supernatant) and dilute with water in 100ml flask. 7. Measure the yield of 6-APA with HPLC by calculating peak area.

Specifications

Product Name

Immobilized Penicillin G acylase, CAS No.9014-06-6

Sinónimos

Penicillin G acylase (Immobilized) | penicillin amidase | penicillin acylase | Penicillin Amidohydrolase | pac

Grado

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Especificaciones y pureza

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥500 U/g wet weight

Bioactividad

≥500 U/g wet weight

Número de adhesión

P06875

CAS

9014-06-6

Número de la Comisión de Enzimas

EC 3.5.1.11

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

≥500 U/g wet weight

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Definición de unidad

One unit (U) represents the amount of enzyme required for adding 1µ mol NaOH per min to adjust pH with degradation of Pen G to 6-APA in 50mM K-phosphate, pH 8.0, at 28℃.