Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin′s A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues. This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker′s yeast.
Product description
Human insulin is a polypeptide hormone secreted by pancreatic β-cells. Insulin plays a crucial role in numerous cellular activities, such as promoting the transport of glucose and amino acids, enhancing anabolism and reducing catabolism, stimulating cell growth, and more. Insulin is a common additive in serum-free mammalian cell culture media. Human insulin is produced through fermentation, separation, high-level purification, crystallization, and drying from Escherichia coli containing a gene that efficiently expresses human insulin. Human insulin is a protein consisting of 51 amino acid residues, with a molecular weight of 5808 Da.
Usage
Before use, dissolve it in a solution made with hydrochloric acid solution (10mM) to obtain a concentration of 1~2mg insulin per 1ml.
Purpose
It is only for R&D purposes and cannot be used for clinical treatment or consumption
Advantages and Specific
No animal-derived sources: The production process is free from exogenous viral contamination and does not utilize any animal-derived raw materials.
Stable quality: Mass production ensures stable and continuous batch production; there is no difference between product batches, and the quality remains stable.
High purity: high specific activity; host protein residuals are below the limit requirements for biological products.
Freeze-dried powder: Easy to store and transport.
Application
Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate.
Recombinant Human Insulin has been used in the media for adipogenic induction of mesenchymal stem cells.
It has been used as a supplement in the cell culture media of airway epithelial cells.
It has been used in the culture media for differentiation of cells, GLUT4 (glucose transporter type 4) translocation assay and to study interaction of insulin with insulin receptor via surface plasmon resonance.
