Kappa light chain Rat mAb (PE-Cy7) - Primary antibody, specific to Kappa light chain

Cat. No.: Ab190253

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

187.1 (HB-58)

Conjugation

PE-Cy7

Descripción general

Rat anti Mouse Kappa light chain Antibody, Monoclonal (187.1 (HB-58)), could be used for Flow Cytometry.

Specifications

Product Name

Kappa light chain Rat mAb (PE-Cy7) - Primary antibody, specific to Kappa light chain

Sinónimos

HCAK 1 antibody | HCAK1 antibody | Ig kappa chain C region antibody | IGKC antibody | IGKCD antibody | Immunoglobulin InV antibody | Immunoglobulin kappa constant antibody | Immunoglobulin kappa constant region antibody | Immunoglobulin kappa light chain

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Rat

Especificidad

Kappa light chain

Inmunógeno

Mouse IgG2b Isotype control antibody clone MPC-11

Control positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugación

PE-Cy7

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

774nm

Ex/Em(nm)

Ex:565nm;Em:774nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Kappa light chain Rat mAb (PE-Cy7) (Ab190253)

Kappa light chain Rat mAb (PE-Cy7) (Ab190253) - Flow Cytometry
Flow cytometry analysis of kappa light chain expression by mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Kappa light chain Rat mAb (PE-Cy7) (Ab190253) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Flow Cytometry: Kappa light chain Rat mAb (PE-Cy7) (Ab190253)

Kappa light chain Rat mAb (PE-Cy7) (Ab190253) - Flow Cytometry
Flow cytometry analysis of kappa light chain expression by mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Kappa light chain Rat mAb (PE-Cy7) (Ab190253) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;