HIBADH Mouse mAb: Detection & Research Applications

HIBADH Mouse mAb is a mouse-derived monoclonal antibody specifically targeting 3-hydroxyisobutyrate dehydrogenase (HIBADH), a mitochondrial enzyme critical to valine catabolism and branched-chain amino acid metabolism.

Product Name

HIBADH Mouse mAb

SKU

Ab350963

Antibody Type

Mouse Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) only

Target Protein

HIBADH (3-Hydroxyisobutyrate Dehydrogenase)

Source Organism

Mouse

What Is HIBADH and Why Does It Matter?

3-Hydroxyisobutyrate dehydrogenase (HIBADH) is a mitochondrial NAD⁺-dependent enzyme encoded by the HIBADH gene. It catalyzes the reversible oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde, a key step in the catabolic pathway of the branched-chain amino acid valine.

HIBADH activity is closely linked to mitochondrial function and energy metabolism. Emerging research has implicated HIBADH in various physiological and pathological contexts, including cancer metabolism, obesity, and type 2 diabetes, making it a target of increasing scientific interest. Reliable antibody tools such as HIBADH Mouse mAb are essential for characterizing expression levels and subcellular localization of this enzyme across experimental models.

Product Description and Formulation

HIBADH Mouse mAb (SKU: Ab350963) is a monoclonal antibody raised in mouse against the human HIBADH protein. The antibody is supplied in phosphate-buffered saline (PBS) only, without additional carrier proteins or preservatives, which makes it compatible with a broad range of downstream conjugation and assay workflows.

The PBS-only formulation is particularly advantageous for researchers requiring direct labeling, biotin conjugation, or coupling to solid supports, as extraneous proteins such as BSA or azide preservatives are absent. Users should store the antibody according to the recommended conditions provided on the product datasheet and avoid repeated freeze-thaw cycles to maintain optimal activity.

Research Applications

Monoclonal antibodies targeting HIBADH, such as this mouse mAb, are broadly applicable in standard immunological and biochemical techniques. Typical applications include:

• Western Blot (WB): Detection of HIBADH protein bands in cell lysates or tissue homogenates under denaturing conditions.
• Immunoprecipitation (IP): Pulldown of HIBADH and associated complexes for interaction studies.
• Immunofluorescence (IF): Visualization of HIBADH subcellular distribution, particularly mitochondrial localization.
• Immunohistochemistry (IHC): Assessment of HIBADH expression in formalin-fixed, paraffin-embedded tissue sections.
• ELISA: Quantitative measurement of HIBADH in biological samples.

Researchers are advised to verify application-specific performance, including optimal dilution titers, by consulting the product datasheet or contacting Aladdin Scientific technical support.

Handling, Storage, and Quality Considerations

To ensure optimal performance of HIBADH Mouse mAb, the following general best practices apply to monoclonal antibodies formulated in PBS:

• Storage temperature: Store at –20 °C for long-term storage, or at 4 °C for short-term use (up to four weeks).
• Aliquoting: Prepare single-use aliquots upon receipt to minimize freeze-thaw cycles, which can lead to aggregation and loss of binding activity.
• Sterile handling: Use aseptic technique when working with the stock solution to prevent microbial contamination.
• Concentration verification: Measure antibody concentration by absorbance at 280 nm (A280) before use if accurate quantification is needed for conjugation reactions.

Always consult the current product datasheet for lot-specific storage conditions and expiration information.

Frequently asked questions

What is the host species and antibody format of HIBADH Mouse mAb?

HIBADH Mouse mAb (Ab350963) is a monoclonal antibody produced in mouse (Mus musculus). It is a monoclonal antibody (mAb), offering high specificity and batch-to-batch consistency compared to polyclonal alternatives.

Why is the PBS-only formulation important for certain experiments?

The absence of carrier proteins such as BSA and preservatives such as sodium azide in the PBS-only formulation means the antibody can be directly conjugated to enzymes, fluorophores, or solid supports without interference. This is critical for ELISA capture antibody development, direct labeling workflows, and multiplexed assay formats.

What biological pathway does HIBADH participate in?

HIBADH encodes 3-hydroxyisobutyrate dehydrogenase, a mitochondrial enzyme that participates in the catabolism of the branched-chain amino acid valine. Specifically, it catalyzes the NAD+-dependent oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde, feeding carbon intermediates into the tricarboxylic acid (TCA) cycle.

How should I determine the optimal working dilution for HIBADH Mouse mAb?

Optimal working dilutions vary by application (WB, IHC, IF, ELISA) and by the specific experimental system used. We recommend starting with the dilution ranges suggested on the product datasheet and performing a titration experiment with positive control samples to identify the dilution that provides maximum signal with minimal background.

References

1. Rougraff PM, Bhatt B, Crochet M, Paxton R, Harris RA. Purification and characterization of 3-hydroxyisobutyrate dehydrogenase from rabbit liver. J Biol Chem. 1988;263(1):327-331.
2. Kasahara T, Shimizu A, Kosuge T, et al. Identification and characterization of HIBADH as a mitochondrial enzyme involved in valine catabolism. Biochem Biophys Res Commun. (year not confirmed in source material).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.