Recombinant Human FARSB Protein: Properties & Applications

Recombinant Human FARSB Protein is a key research reagent representing the beta subunit of the heterodimeric phenylalanyl-tRNA synthetase (PheRS), an essential enzyme responsible for charging tRNA with phenylalanine during cytoplasmic protein biosynthesis in human cells.

Protein Name

Phenylalanyl-tRNA Synthetase Beta Subunit (FARSB)

SKU

rp367642

Protein Type

Recombinant Human Protein

Biological Function

Beta subunit of the cytoplasmic phenylalanyl-tRNA synthetase; catalyzes aminoacylation of tRNA-Phe

Gene Name

FARSB (also known as FARSLB, PHEBETA, PheRS-beta)

Supplier

Aladdin Scientific

Introduction to FARSB and Its Biological Role

The FARSB gene encodes the beta subunit of cytoplasmic phenylalanyl-tRNA synthetase (PheRS), a class II aminoacyl-tRNA synthetase (aaRS). AaRS enzymes are essential components of the translational machinery, catalyzing the attachment of specific amino acids to their cognate transfer RNAs (tRNAs) — a process called aminoacylation or tRNA charging — which is a prerequisite for accurate ribosomal protein synthesis.

Cytoplasmic PheRS is an alpha2/beta2 heterotetramer, composed of two FARSA (alpha) subunits and two FARSB (beta) subunits. While the alpha subunit harbors the catalytic aminoacylation domain, the beta subunit plays critical structural and regulatory roles, including anticodon-binding functions and overall complex stability. Dysregulation or mutation of FARSB has been associated with neurodevelopmental disorders and other human diseases, making it a clinically relevant research target.

Biochemical Properties of Recombinant Human FARSB Protein

Recombinant Human FARSB Protein (SKU: rp367642) is produced using recombinant expression systems to ensure high purity and biological activity suitable for in vitro research applications. As a recombinant protein reagent, it enables researchers to study FARSB function, interactions, and regulation in controlled experimental settings without the variability inherent to native tissue extraction.

Key biochemical characteristics of FARSB include its membership in the class II aaRS superfamily, defined by three conserved sequence motifs. The protein participates in multi-synthetase complex (MSC) assembly, a macromolecular structure that is increasingly recognized for its roles beyond translation, including signaling and immune response modulation. Researchers should consult the product datasheet (absea#PP-5477) for specific details on host expression system, tag, purity grade, formulation, and recommended storage conditions.

Research Applications of Recombinant Human FARSB Protein

Recombinant Human FARSB Protein serves as a versatile tool in multiple research contexts:

• Biochemical Assays: Used as a substrate or binding partner in enzyme activity assays, binding studies, and inhibitor screening to investigate aaRS biology and identify potential therapeutic targets.
• Structural Biology: Suitable as a component for crystallography, cryo-EM, or biophysical interaction studies aimed at resolving the structure of human cytoplasmic PheRS or its subcomplexes.
• Antibody Validation: Can serve as a positive control antigen for validating anti-FARSB antibodies in Western blot, ELISA, and immunoprecipitation experiments.
• Disease Mechanism Research: Facilitates investigation of FARSB-linked pathologies, including studies on mutations associated with neurodevelopmental and metabolic disorders.
• Multi-Synthetase Complex Studies: Supports research into the assembly, composition, and non-canonical functions of the human MSC, which is an emerging area of therapeutic interest.

This recombinant protein is intended for research use only and is not approved for diagnostic or therapeutic applications in humans.

Storage, Handling, and Quality Considerations

Proper handling of Recombinant Human FARSB Protein is essential to maintain its integrity and bioactivity. General best practices for recombinant proteins apply:

• Storage: Aliquot upon receipt to minimize freeze-thaw cycles. Store according to the conditions specified in the product datasheet (absea#PP-5477). Typical storage for recombinant proteins is at -20°C or -80°C in the presence of a carrier protein or stabilizing buffer.
• Reconstitution: If supplied lyophilized, reconstitute with the recommended buffer as described in the datasheet, and allow the solution to equilibrate gently without vortexing.
• Quality Control: Aladdin Scientific applies rigorous QC measures including SDS-PAGE, and activity or binding assays where applicable, to confirm purity and functional integrity of recombinant proteins before release.
• Avoid Repeated Freeze-Thaw: Repeated freeze-thaw cycles can lead to protein aggregation and loss of activity. Single-use aliquots are strongly recommended.

For specific formulation details, concentration, purity specifications, and lot-specific QC data, always refer to the official product datasheet provided with your shipment or available upon request from Aladdin Scientific.

Frequently asked questions

What is the function of the FARSB protein in human cells?

FARSB encodes the beta subunit of the cytoplasmic phenylalanyl-tRNA synthetase (PheRS), which catalyzes the aminoacylation of tRNA with phenylalanine, an obligatory step in cytoplasmic protein synthesis. The beta subunit contributes to anticodon recognition and structural stability of the heterotetramer complex. It also participates in the assembly of the multi-synthetase complex (MSC), which has emerging non-translational signaling roles.

What research applications is Recombinant Human FARSB Protein (rp367642) suitable for?

Recombinant Human FARSB Protein is suitable for a range of in vitro research applications including biochemical enzyme assays, inhibitor screening, antibody validation (Western blot, ELISA, immunoprecipitation), structural biology studies (crystallography, cryo-EM), and investigation of FARSB-associated disease mechanisms. It is also used in studies of multi-synthetase complex assembly and non-canonical aaRS functions.

How should Recombinant Human FARSB Protein be stored to maintain its activity?

To maintain bioactivity, aliquot the protein upon receipt to avoid repeated freeze-thaw cycles, which can cause aggregation and activity loss. Store under the conditions specified in the product datasheet (absea#PP-5477), typically at -20°C or -80°C. If the product is lyophilized, reconstitute gently with the recommended buffer and avoid vigorous vortexing.

Is Recombinant Human FARSB Protein approved for clinical or diagnostic use?

No. Recombinant Human FARSB Protein (rp367642) from Aladdin Scientific is intended for research use only (RUO). It has not been evaluated or approved for use in diagnostic procedures or therapeutic applications in humans or animals.

References

1. Ibba M, Soll D. Aminoacyl-tRNA synthesis. Annual Review of Biochemistry. 2000;69:617-650. doi:10.1146/annurev.biochem.69.1.617
2. Guo M, Schimmel P. Essential nontranslational functions of tRNA synthetases. Nature Chemical Biology. 2013;9(3):145-153. doi:10.1038/nchembio.1158
3. Ray PS, Fox PL. A post-transcriptional pathway represses monocyte VEGF-A expression and angiogenic activity. EMBO Journal. 2007;26(14):3360-3372. doi:10.1038/sj.emboj.7601774
4. Aladdin Scientific. Recombinant Human FARSB Protein, SKU rp367642. Product Datasheet absea#PP-5477.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.