Dissolve 10.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for15 minutes at 121ºC (15psi).
Component
g/liter
Ammonium Chloride
1
Potassium Phosphate mono
3
Sodium Chloride
0.5
Sodium Phosphate dibasic
6
Total: 10.5g/Liter
Note: Calcium chloride is not included since it is optional in the preparation of M9. If desired, add 15mg/liter.
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
