Animal model of cardiovascular stasis
Animal model of cardiovascular stasis
The animal model of cardiovascular stasis and obstruction syndrome is characterized by chest tightness and palpitations, heart pain like system, pain leading to the shoulders and back of the inner arm, purple lips and tongue, and fine or knotty pulse, and so on. At present, the common modeling methods for animal models of cardiac blood stasis and obstruction include canine coronary artery ligation.
Principle
The basic principle of canine coronary artery ligation method to establish an animal model of cardiac blood stasis is to ligate with a thread or compress a branch of the coronary artery with an airbag in animals such as dogs or rats, resulting in experimental myocardial infarction or myocardial ischemia.
Appliance
The animal model of cardio-blood stasis and obstruction syndrome can be used for the study of cardio-blood stasis and obstruction syndrome and the study of related prescription drugs.
Operation method
Establishment of an animal model of cardiac blood stasis syndrome by canine coronary artery ligation method
Principle
The basic principle of canine coronary artery ligation method to establish an animal model of cardiac blood stasis is to ligate with a thread or compress a branch of the coronary artery with an airbag in animals such as dogs or rats, resulting in experimental myocardial infarction or myocardial ischemia.
Materials and Instruments
Laboratory animals: Move Canine coronary artery ligation method to establish an animal model of cardiac blood stasis is as follows: Caveat 1. Pay attention to the selection of the ligation site, the further away from the origin of the coronary artery, the finer the coronary vessels ligated, the lower the mortality rate of the animals. For more product details, please visit Aladdin Scientific website.
① Dog.
Equipment:
① A set of medical surgical instruments.
Reagents:
① Sodium pentobarbital.
A. After anesthesia with sodium pentobarbital, the dog is fixed in the supine position, the trachea is incised, and an artificial respirator is connected to carry out artificial respiration.
B. The chest is opened at the left 4th intercostal space, the heart is exposed, the pericardium is incised, and the ligature point is chosen at the upper 1/3 (1-2 cm from the beginning of the anterior descending branch of the left coronary artery), or 1/2, or lower 1/3 (above the apical branch), causing myocardial infarction. (above the apical branch), a ligature point is selected, and a wire is threaded through it to cause myocardial infarction, which is called one-stage ligation.
C. In order to minimize the occurrence of arrhythmic deaths in animals, the two-stage ligation method is often used. That is, in a ligation point wear two silk threads, put the No. 5 needle in the knot and the coronary artery parallel position, ligation of the first silk thread (first ligation), the needle and the anterior descending branch of the coronary artery with ligation, withdraw the needle, and then ligation of the second silk thread after 30 minutes (second ligation).
D. If the epicardial electrodes are not carried out, you can suture the pericardium, sew up the chest wall, and let it resume natural respiration, and then connect to the electrocardiographic oscilloscope to observe the changes of electrocardiography. observe the electrocardiographic changes.