Sieving agarose gel electrophoresis experiments

Summary

Sieve agarose gels spread and electrophoresis like regular agarose gels, but they can resolve small fragments of DNA as well as non-denaturing polyacrylamide gels.

Operation method

electrophoresis experiment

Materials and Instruments

DNA
TAE Agarose
Electrophoresis

Move

1. Melt 3% to 5% low melting point sieved agarose in TAE electrophoresis buffer. Pour the gel into a regular agarose gel apparatus.

2. Add samples and electrophoresis as for normal agarose gels. (For 2% gels bromophenol blue migrates to approximately 50 bp)
3. Separate fragments in low melting point agarose. (Yields for fragments <1,000 bp are similar to those recovered with regular low-melting-point agarose, with maximum separation achieved for fragments <500 bp).

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