Observation experiment on the diuretic effect of drugs
Observation experiment on the diuretic effect of drugs
NA is an a receptor agonist, a larger dose can constrict renal blood vessels, reduce renal blood flow, resulting in a decrease in urine output; furosemide is a highly effective diuretic, through the inhibition of renal tubular medullary collaterals of the ascending branch of the thick segment of the Na-K-2Cl synergistic transport system to increase the concentration of Na, K and Cl in the tubular fluid, so that the medullary osmotic gradient can not be established to reduce the dilution and concentration of the kidney, and ultimately discharged a large number of nearly isotonic urine. The final result is the discharge of a large amount of nearly isotonic urine.
Operation method
osmolality
Principle
NA is an a receptor agonist, a larger dose can constrict renal blood vessels, reduce renal blood flow, resulting in a decrease in urine output; furosemide is a highly effective diuretic, through the inhibition of renal tubular medullary collaterals of the ascending branch of the thick segment of the Na-K-2Cl synergistic transport system to increase the concentration of Na, K and Cl in the tubular fluid, so that the medullary osmotic gradient can not be established to reduce the dilution and concentration of the kidney, and ultimately discharged a large number of nearly isotonic urine. The final result is the discharge of a large amount of nearly isotonic urine.
Materials and Instruments
Rabbit Move 1. 1 male rabbit weighing about 2 Kg was gavaged with 50 ml of 5% dextrose saline 0.5 h before the experiment to increase the water load. For more product details, please visit Aladdin Scientific website.
Glucose Saline NA Furosemide Liquid Paraffin Silver Nitrate Standard Potassium Chromate Solution
Animal scales Rabbit tables Rabbit cords Catheter #8 Graduated test tubes Beakers Measuring cylinders Rabbit cotters Stomach tubes Tape Syringes Straws Titration tubes Conical flasks
2. The rabbit was weighed, anesthetized and fixed.
3. Apply liquid paraffin to the tip of the catheter, remove the foreskin, lift up the penis, and slowly insert it through the urethral opening for about 7-9 cm into the bladder and squeeze out the excess urine.
4. 5% dextrose saline was given as a continuous drip from a vein at the ear margin of the rabbit, and the rabbit was observed for the character of the urine until the urine was clear and started to be collected every 5 minutes for 3 consecutive times, and the average value was taken as the level of urine volume before administration.
5. Slowly give 0.1% NA 1 ml/Kg intravenously, collect the urine volume for 15 minutes as above, and observe the change of urine volume of the rabbits.
6. Give another 1% furosemide 0.5 ml/Kg, collect the urine volume for 15 minutes as above, and observe the change of urine volume of rabbits.
7. Take the normal. After administration of NA. Titrate the urine after administration of furosemide to find out the content of Cl-. Record the results of the experiment and plot the graph.
Notes:
1. Be sure to allow the rabbit to water load completely.
2. Before each recording of urine output, gently press the rabbit's lower abdomen with your hand to drain the bladder.
4. Urine should flow 1-3 minutes after administration. If no urine is present, the catheter can be gently turned and urine will flow.