Simian vacuolar virus type 1 vector

Summary

Vacuolar viruses (FV) are non-pathogenic retroviruses that efficiently and safely transfer genes to different types of cells in different species. These viruses have a unique replication mechanism that is different from that of other retroviruses, which is an advantage of vacuolar viruses in mediating gene transfer. Their reverse transcription occurs late in the viral life cycle. At this point, at least 20% of the virus particles contain infectious double-stranded DNA genomes Author: T. Friedman et al.

Operation method

Preparation of SFV-1 Vector

Move

Preparation of SFV-1 Vector Material

reagents
CaCl2 (2m o l /L )

Chlorine (50m m o l /L)

Complete DMEM medium with 10 % FBS, 2 m m o l / L L-glutamine, l O O U / m l penicillin,

100ug/ml streptomycin

Fetal Bovine Serum (F B S )

2XHEPES buffer (HBS; 50 mmol/L HEPES, O.78 mmol/L Na2 HPO4, and 273 mmol/L NaCl)

H a n k balanced salt solution (H B S S )

Human embryonic kidney fibroblast-derived 293T cells

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