Reagent Specifications, Grading and Purity

HPLC Gradient Grade Solvents (≥99.9% Purity)

Optimized for High-Resolution Gradient Chromatography


Aladdin Scientific offers a curated selection of HPLC Gradient Grade solvents with ≥99.9% purity, specially formulated for use in gradient elution HPLC workflows. These ultra-clean solvents are designed to deliver baseline stability, excellent reproducibility, and minimal UV interference, even during shallow or extended gradients.


Key Features & Benefits

· Gradient-Grade Certified (≥99.9% Purity)
Delivers reliable peak performance across the entire mobile phase gradient without introducing background drift or chemical interference.

· Low UV Absorbance at Critical Wavelengths
Spectrally clean at 190–254 nm for sensitive UV and diode-array detection, ensuring accurate quantification and detection of low-abundance analytes.

· Low Non-Volatile Residue & Particulates
Prevents detector fouling and column damage. Ideal for long-term use in high-throughput analytical systems.

· Batch-to-Batch Consistency
Each lot undergoes rigorous testing and is accompanied by a Certificate of Analysis (CoA) to confirm chromatographic suitability.

· Filtration
Final filtration through 0.2 µm membranes ensures a particle-free solution ready for direct use in HPLC systems.


Available Solvents – HPLC Gradient Grade

Product Name

Catalog Example

Purity

UV Cutoff (nm)

Formats

Acetonitrile, HPLC Gradient Grade

[e.g. ACN-GR-99.9]

≥99.9%

~190 nm

1L, 2.5L, 4L

Methanol, HPLC Gradient Grade

[e.g. MeOH-GR-99.9]

≥99.9%

~205 nm

1L, 2.5L, 4L

Water, HPLC Gradient Grade

[e.g. H2O-GR-99.9]

≥99.9%

~190 nm

1L, 2.5L, 4L

Isopropanol (IPA), HPLC Gradient Grade

[e.g. IPA-GR-99.9]

≥99.9%

~205 nm

1L, 2.5L

(Custom packaging and bulk volumes available upon request.)


Applications

· Gradient-based reversed-phase HPLC

· Analytical method development

· Pharmaceutical QC and R&D

· Food, beverage, and environmental testing

· Cosmetic and chemical impurity analysis


Why Choose Aladdin Scientific HPLC Grade Solvents?

· High-performance formulation for stable baselines and sharp peak symmetry

· Trusted by laboratories conducting regulated and non-regulated HPLC workflows

· Compatible with all major chromatography platforms (Agilent, Shimadzu, Waters, Thermo, etc.)

· ISO-certified manufacturing and rigorous traceability


Supporting Documentation

· Certificate of Analysis (CoA) included with every batch

· Material Safety Data Sheet (MSDS) available online

· Method suitability statement for gradient HPLC


Search-Friendly Keywords (SEO-Optimized)

· HPLC gradient grade solvents

· HPLC acetonitrile methanol ≥99.9%

· Gradient grade mobile phase solvents

· UV-transparent HPLC solvents

· High-purity solvents for reversed-phase chromatography

 

View all HPLC Gradient Grade Solvents (≥99.9% Purity) Products

Categories: Reagent Specifications, Grading and Purity

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "HPLC Gradient Grade Solvents (≥99.9% Purity)" Aladdin Knowledge Base, updated 23 jul 2025. https://www.aladdinsci.com/us_es/faqs/hplc-gradient-grade-solvents-en.html

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