KP372-1 - 10mM in DMSO , CAS No.1374996-60-7

CAS: 1374996-60-7 Cat. No.: K655617 Fórmula: C₂₀H₈N₁₂O₂ Peso molecular: 448.4 Número EC: 808-876-3 PubChem CID: 49835991

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

KP372-1 is an Akt inhibitor that inhibits proliferation and induces apoptosis and anoikis. KP372-1 is also an NQO1 redox cycling agent that causes DNA damage (including DNA breakage) by generating ROS. KP372-1 can be used in cancer research (such as head and neck squamous cell carcinoma (HNSCC) and pancreatic cancer) .

In Vitro

KP372-1 (0.0625, 0.125, 0.25, 0.5, 1.0 µM; 48 h) inhibits growth of JMARc42 and Tu167c2 cells with IC 50 s of 200 and 100 nM, respectively. KP372-1 (125 nM; 24 h) induces Tu167c2 cells apoptosis and induces anoikis in the JMARc42 cells. KP372-1 (125 nM; 30 min) blocks Akt, thereby decreasing the phosphorylation of the S6 ribosomal protein in both Tu167 and JMAR cells. KP372-1 (0.250, 0.5, 1.0 µM; 30 min) inhibits Akt kinase activity with an IC 50 of 250 nM in JMAR cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: JMARc42 and Tu167c2 cells Concentration: 0.0625, 0.125, 0.25, 0.5, 1.0 µM Incubation Time: 48 h Result: Showed antiproliferative activity. Apoptosis AnalysisCell Line: Tu167c2 and JMARc42 cells Concentration: 125 nM Incubation Time: 24 h Result: Induced approximately 90% of cells apoptosis. Western Blot AnalysisCell Line: Tu167 and JMAR cells Concentration: 125 nM Incubation Time: 30 min Result: Induced a small but consistent decrease in Akt phosphorylation with a concomitant marked decrease in S6 phosphorylation. Inhibited the EGF induced phosphorylation of Aktser473 in Tu167 and AktThr308 in JMAR. Western Blot AnalysisCell Line: JMAR cells Concentration: 0.250, 0.5, 1.0 µM Incubation Time: 30 min Result: Significantly blocked Akt kinase activity in a dose-dependent fashion, with an IC 50 of 250 nM.

In Vivo

KP372-1(10, 20 mg/kg; i.v.; single daily for 33 days) induces NADH oxidation and impairs tumor growth in vivo without apparent toxicity. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Nude mice (H1299 xenografts model). Dosage: 10, 20 mg/kg Administration: Tailvein injection; single daily for 33 days Result: Affected tumor metabolism and suppressed tumor growth.

Specifications

Especificaciones y pureza

10mM in DMSO

Mecanismos bioquímicos y fisiológicos

KP372-1 is an Akt inhibitor that inhibits proliferation and induces apoptosis and anoikis. KP372-1 is also an NQO1 redox cycling agent that causes DNA damage (including DNA breakage) by generating ROS. KP372-1 can be used in cancer research (such as head

Condiciones de almacenamiento de almacenamiento

Store at -80°C

Enviado en

Dry ice packs + Cold packs

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Nombres e identificadores

Isómeros SMILES

C1=CC=C2C(=C1)C3=NC4=NN=NN4N=C3C2=O.C1=CC=C2C(=C1)C3=NN4C(=NN=N4)N=C3C2=O

WGK Alemania

3

PubChem CID

49835991

Peso molecular

448.4

Preguntas frecuentes

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1374996-60-7, the molecular formula is C20H8N12O2, and the molecular weight is 448.4 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.