α-L-Arabinofuranosidase, CAS No.9067-74-7

CAS: 9067-74-7 Cat. No.: L1438303 Número EC: 232-957-7

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Aspergillus niger; ~300 U/mL; ≥32 U/mg protein

Accession #

P79019,P42254,P42255

Bioactivity

≥32 U/mg protein

Descripción general

High purity α-L-Arabinofuranosidase (Aspergillus niger) for use in research, biochemical enzyme assays and analytical testing applications. Applications in carbohydrate and biofuels research.

PROPERTIES

1. ELECTROPHORETIC PURITY:

• Single major band on SDS-gel electrophoresis (MW = 62,000)
• Single major band on isoelectric focusing (pI < 3.0)

2. SPECIFICITY:

Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans.Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate.

3. PHYSICOCHEMICAL PROPERTIES:

Recommended conditions of use are at pH 4.0-4.5 and up to 60°C.

• pH Optima: 4.0
• pH Stability: 3.5-5.0 (> 75% control activity after 16 h at 4°C)
• Temperature Optima: 40°C (10 min reaction)
• Temperature Stability: up to 60°C (> 75% control activity after 15 min incubation at temperature)

4. STORAGE CONDITIONS:

The enzyme is supplied as a suspension in 3.2 M ammonium sulphate containing 0.02% (w/v) sodium azide and should be stored at 4°C. For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 4.0. Swirl to mix the enzyme immediately prior to use.

5. EXPERIMENTAL DATA:

Specifications

Product Name

α-L-Arabinofuranosidase, CAS No.9067-74-7

Sinónimos

exo-α-Arabinofuranosidase | non-reducing end alpha-L-arabinofuranosidase | alpha-L-arabinofuranosidase | arabinofuranosidase | arabinosidase | axhA | abfA | abfB

Grado

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Especificaciones y pureza

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Aspergillus niger; ~300 U/mL; ≥32 U/mg protein

Bioactividad

≥32 U/mg protein

Número de adhesión

P79019, P42254, P42255

CAS

9067-74-7

Número de la Comisión de Enzimas

EC 3.2.1.55

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

from Aspergillus niger; ~300 U/mL; ≥32 U/mg protein

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Definición de unidad

One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one μmole ofp-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4.0 at 40°C.