GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal
Especies hospedadoras
Mouse
Especificidad
Lamin A
Inmunógeno
Recombinant protein corresponding to Human Lamin A
Control positivo
Flow Cytometry: HeLa cells ICC/IF: HeLa cells
Conjugación
AF488
Excitación (nm)
Blue Laser(488nm)
Emisión (nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grado
ExactAb™, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Imágenes
Lamin A/C Mouse mAb (AF488) (Ab219214) - IF/ICC IF analysis of Lamin A/C (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (AF488) (Ab219214) at a dilution of 1/1000 in blocking buffer for 2 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Lamin A/C Mouse mAb (AF488) (Ab219214) - Flow Cytometry Flow cytometry analysis of Lamin A/C expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Lamin A/C Mouse mAb (AF488) (Ab219214) (Red) at a dilution of 1/900 for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
Flow Cytometry
1/100-1/1000
IF/ICC
1/100-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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