GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein
EnzymoPure™, Recombinant, lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Product introduction
Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Application
The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
Specifications
Product Name
Luciferase from Photinus pyralis (firefly) , CAS No.61970-00-1
EnzymoPure™, Recombinant, lyophilized powder, ≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein
Fuente
大肠杆菌
Nota
Packaging 1mg refers to the quality of the protein.
CAS
61970-00-1
Número de la Comisión de Enzimas
1.13.12.7
Tipo de molécula
Enzyme
Almacenamiento y envío
Concentración
lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein
Condiciones de almacenamiento de almacenamiento
Store at -80°C
Enviado en
Dry ice packs + Cold packs
Definición de unidad
One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
1.Wang Zhengwei, Lin Jiani, Tan Guisen, Pan Tingzheng, Zhang Hongtian, Liu Jiangang. (2025) miR-1270 suppresses glioblastoma development by transcriptional inhibition of nuclear factor IX. MOLECULAR BIOLOGY REPORTS, 52 (1):(1-10). [PMID:40815328][10.1007/s11033-025-10915-3]
2.Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo. (2025) Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation. PLANT CELL AND ENVIRONMENT, [PMID:40947544][10.1111/pce.70180]
Calculadoras de soluciones
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Reseñas
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Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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