Carrier Free,Validated,for conjugation,5mg/mL Carrier Free,for conjugation,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
The SpyTag/SpyCatcher system is a convenient protein coupling tool for irreversible peptide-protein ligation. It is ideal for binding, labeling, immobilization and creating new kinds of protein architectures.
Peptide interaction with proteins is usually weak. SpyTag is a genetically encoded peptide (SpyTag003 sequence RGVPHIVMVDAYKRYK) that forms a spontaneous amide bond upon binding its genetically encoded partner SpyCatcher. SpyTag003 reacts with SpyCatcher under a wide range of conditions.
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and high quantum efficiency. APC has an excitation peak at 650 nm and an emission peak at 660 nm.
SpyCatcher003 (S49C) has a unique cysteine for precise labeling with APC via maleimide chemistry for instance. We have successfully used this APC labelled SpyCatcher003 (S49C) protein to conjugate a recombinant OKT3 antibody that already has a SpyTag. The result APC labelled OKT3 antibody was successfully used for flow cytometry.
Guideline for Use
1. The concentration is determined by absorption = 73 at 650 nm for a 1% solution for only those APC molecules to which at least one molecule of SpyCatcher003 (S49C) is bound.
2. Add 2mg APC labelled SpyCatcher003 (S49C) protein per mg of Spytag modified antibody. Mix and incubate for 2 hours at room temperature or overnight at 4°C. The labeled antibody can be directly tested by flow cytometry. The amount of protein can be scaled proportionally.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ26F0332474 | Certificate of Analysis | Mar 05, 2026 | rp218715 |
| ZJ26F0332473 | Certificate of Analysis | Mar 05, 2026 | rp218715 |
| ZJ26F0332472 | Certificate of Analysis | Mar 05, 2026 | rp218715 |
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