DBCO-modified Allophycocyanin (DBCO-APC) produced by Aladdin is a ready-to-use click chemistry conjugation reagent, prepared by covalently modifying high-purity Allophycocyanin with DBCO-PEG5-NHS ester. This product enables rapid and efficient copper-free SPAAC (strain-promoted azide-alkyne cycloaddition) with azide-modified proteins, antibodies, oligonucleotides, polymers, or other molecules under mild conditions.
Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm. APC is a large chemically-crosslinked molecule (~104KD) used in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. DBCO (dibenzocyclooctyne) is a cyclooctyne that undergoes efficient, copper-free click chemistry with azides under mild conditions. This reaction features high selectivity, fast kinetics, and excellent biocompatibility, making it suitable for live-cell and in vivo applications. The introduction of a polyethylene glycol (PEG) spacer enhances the reagent’s solubility, stability, and biocompatibility, reduces non-specific binding, and prolongs circulation half-life. DBCO-APC combines the advantages of Allophycocyanin and DBCO groups, enabling efficient, copper-free conjugation to azide-modified proteins, peptides, and oligonucleotides, and is well-suited for flow cytometry, imaging, and assay development.
This DBCO-APC product is purified by size exclusion chromatography to remove unconjugated molecules, ensuring optimal staining performance.
Usage Suggestion
Storage conditions: The lyophilized powder should be stored dry and protected from light at 2–8°C for long-term preservation. Do not freeze. After reconstitution, the solution is recommended to be stored at 4°C for no more than 1 week. Phycobiliproteins are sensitive to freeze-thaw cycles. Avoid exposure to heat and light.
Copper-Free Click Reaction:
1. For azide-modified antibody labeling as an example: DBCO-APC is recommended at a concentration of 5 mg/mL, with a labeling mass ratio of IgG:APC = 1:2. Mix DBCO-APC with the azide-modified protein or antibody.
2. Incubate for 2–4 hours at room temperature, or overnight at 4°C.
3. Depending on your application, the conjugated protein can be used immediately or purified by size exclusion chromatography.
40、内部备注：该产品原料为[C388022] 交联别藻蓝蛋白和[D338360] DBCO-NHCO-PEG5-NHS ester
ZJ26F0535839