LY2409881 , CAS No.946518-61-2

CAS: 946518-61-2 Cat. No.: L647032 Peso molecular: 485.04 PubChem CID: 16720762

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

LY2409881 is a selective IκB kinase β ( IKK2 ) inhibitor with an IC 50 of 30 nM.

In Vitro

LY2409881 is an IKK2 inhibitor that inhibits TNFα-induced activation of NF-κB. By in vitro kinase assay, LY2409881 potently inhibits IKK2, with an IC 50 of 30 nM. In contrast, the IC 50 for IKK1 and other common kinases is at least one log higher. The specificity of LY2409881 for NF-κB signaling is further studied in a cell-based assay, by examining the effect of LY2409881 in the TNFα-dependent antiapoptosis function. TNFα is a well-characterized upstream stimulus of NF-κB. In the ovarian cancer cell line SKOV3, LY2409881 demonstrates moderate cytotoxicity, whereas TNFα at 10 ng/mL does not cause any cytotoxicity. In contrast, coadministration of LY2409881 and TNFα results in markedly higher cell killing compared with LY2409881. This is because TNFα-dependent activation of antiapoptotic signals mediated by NF-κB is blocked by LY2409881, while the proapoptotic TNF receptor-associated death domain (TRADD) and FAS-associated death domain (FADD) cascade pathways activated by TNFα are not affected by LY2409881. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

A well-established xenograft model of DLBCL is used to confirm the activity of LY2409881 in vivo. SCID-beige mice implanted with LY10 cell-derived tumors are given intraperitoneal injections of LY2409881 twice weekly at three different doses: 50, 100, and 200 mg/kg. The treatments are well tolerated, resulting in no death or severe morbidity of the mice. The average tumor volume is graphed as a function of time for each treatment group. The rates of tumor volume growth of the treatment groups are all significantly slower than the untreated control group (P≤0.01) . MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:IKK2 30 nM (IC 50 )

Specifications

Mecanismos bioquímicos y fisiológicos

LY2409881 is a selective IκB kinase β ( IKK2 ) inhibitor with an IC 50 of 30 nM.

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

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Tipo de acción

INHIBITOR

Nombres e identificadores

• Isómeros SMILES: CN1CCN(CC1)CCCNC2=NC=C(C(=N2)C3=CC4=C(C=CC=C4S3)C(=O)NC5CC5)Cl
• PubChem CID: 16720762
• Peso molecular: 485.04

Propiedades químicas y físicas

• Solubilidad: DMSO : ≥ 37 mg/mL (76.28 mM)
• Peso molecular: 485.000 g/mol
• XLogP3: 3.900
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 8
• Exact Mass: 484.181 Da
• Monoisotopic Mass: 484.181 Da
• Topological Polar Surface Area: 102.000 Ų
• Heavy Atom Count: 33
• Formal Charge: 0
• Complexity: 661.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1