Lyso-Tracker Red

Cat. No.: L747763

Descripción general

Lyso-Tracker Red (1mM) will solidify on ice or at lower temperatures such as 4℃ and stick to the bottom, walls or lid of the centrifuge tube. It should be thawed completely in a water bath at 20-25℃ prior to use. For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied. They can be ordered from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Preparation of Lyso-Tracker Red working solutiona.Dilute an appropriate amount of Lyso-Tracker Red (1mM) in cell culture medium or other appropriate solutions (e.g., HBSS with Ca2+ & Mg2+) at a ratio of 1:13333-1:20000 to a final concentration of 50-75nM. For example, mix 1µl of Lyso-Tracker Red (1mM) with 20ml or 13.33ml of dilution buffer to obtain the Lyso-Tracker Red working solution. HBSS with Ca2+ & Mg2+ can be ordered from .b.Prewarm the Lyso-Tracker Red working solution at 37℃ before use.Note: The concentration of Lyso-Tracker Red in the working solution can be adjusted appropriately according to the staining results.To minimize the staining background, a lower concentration of Lyso-Tracker Red is preferred as long as an acceptable staining result can be obtained.2.Fluorescent labelling of lysosomea.Remove the cell culture medium, add Lyso-Tracker Red working solution pre-incubated at 37℃, and incubate at 37℃ for 5-60 min.b.Remove the Lyso-Tracker Red working solution and add fresh cell culture medium.c.Examine cells by fluorescence microscope or confocal microscope. If the staining is too weak, increase the concentration of Lyso-Tracker Red or extend the staining time appropriately within the recommended time period.

Specifications

Product Name

Lyso-Tracker Red

Tipo de molécula

Small molecule

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20 ℃ away from light, effective for six months.